Wnt Signaling: Volume 2, Pathway Models

ISBN-13: 9781603274685 / Angielski / Twarda / 2008 / 494 str.

Elizabeth Vincan

Opis

Since their discovery, Wnt signaling molecules have been shown to control key events in embryogenesis, to maintain tissue homeostasis in the adult and, when aberrantly activated, to promote human degenerative diseases and cancer, thus making them a vital area of study. Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 2, Pathway Models, the diverse vertebrate and invertebrate models that have shaped the Wnt signaling field are described, presenting an overview of the unique properties of each organism, like asymmetric cell division in C. elegans and epithelial morphogenesis in Dictyostelium, with respect to studying Wnt/FZD function. As a volume in the highly successful Methods in Molecular Biology series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips. Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field to produce this invaluable two-volume resource."

Spis treści

Part I. Introduction Chapter 1. Evolution of the Wnt pathway. Jennifer C Croce and David R McClay Part II. Dictyostelium Chapter 2. Dictyostelium development: a prototypic Wnt pathway? Adrian J Harwood Chapter 3. Monitoring patterns of gene expression in Dictyostelium by b -galactosidase staining. Adrian J Harwood Chapter 4. Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation. Adrian J Harwood Part III. Cnidarians Chapter 5. Wnt signaling in Cnidarians. Thomas Holstein Chapter 6. Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos Shalika Kumburegama, Naveen Wijesena, and Athula H. Wikramanayake Chapter 7. Detection of expression patterns in Hydra pattern formation. Hans Bode, Tobias Lengfeld, Bert Hombayer and Thomas Holstein Part IV. elegans Chapter 8: Analysis of Wnt signaling pathways during C. elegans postembryonic development. Samantha Van Hoffelen and Michael A. Herman Chapter 9. Wnt signaling during C. Elegans embryonic development. Daniel J Marston, Minna Roh, Amanda J Mikels, Roel Nusse and Bob Goldstein Part V. Drosophila Chapter 10. Function of the Wingless signaling pathway in Drosophila. Foster C Gonsalves and Ramanuj DasGupta Chapter 11. Visualisation of PCP defects in the eye and wing of Drosophila melanogaster Natalia Arbuzova and Helen McNeill Chapter 12. Wingless signaling in Drosophila eye development. Kevin Legent and Jessica Treisman Chapter 13. High-throughput RNAi screen in Drosophila. Ramanuj DasGupta and Foster C Gonsalves Part VI. Sea urchin Chapter 14. Wnt signaling in early sea urchin development. Shalika Kumburegama and Athula H. Wikramanayake Chapter 15. Detecting expression patterns of Wnt pathway components in sea urchin embryos using whole mount in situ hybridization. Joanna M. Bince and Athula H. Wikramanayake Chapter 16. Functional analysis of Wnt signaling in the early sea urchin embryo using mRNA microinjection. Joanna M Bince and Athula H. Wikramanayake Part VII. Zebrafish Chapter 17. Wnt signaling mediates diverse developmental processes in zebrafish. Heather Verkade and Joan K Heath Chapter 18. Determination of mRNA and protein expression patterns in zebrafish. Elizabeth L Christie, Adam C Parslow and Joan K Heath Chapter 19. Manipulation of Gene Expression during Zebrafish Embryonic Development using Transient Approaches, Benjamin M. Hogan, Heather Verkade, Graham J. Lieschke and Joan K. Heath Chapter 20. Neural patterning and CNS functions of Wnt in Zebrafish. Richard Dorsky Part VIII. Xenopus Chapter 21. Studying Wnt signaling in Xenopus. Stefan Hoppler Section A: Methods for studying Wnt signaling in Xenopus embryos Chapter 22. Analysis of gene expression in Xenopus embryos. Danielle L Lavery and Stefan Hoppler Chapter 23. Detection of nuclear b -catenin in xenopus embryos. Francois Fagotto and Carolyn M Brown Chapter 24. Transgenic reporter tools tracing endogenous canonical Wnt signaling in Xenopus. Tinneke Denayer, Hong Thi Tran and Kris Vleminckx Chapter 25. Gain-of-function and Loss-of-function strategies in Xenopus. Danielle L Lavery and Stefan Hoppler Chapter 26. How the mother can help – studying maternal Wnt signaling by antisense-mediated depletion of maternal mRNAs and the host transfer technique. Adnan Mir and Janet Heasman Chapter 27. Inducible gene expression in transient transgenic Xenopus embryos. Grant N