Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology

ISBN-13: 9781316614761 / Angielski / Miękka / 2018 / 956 str.

Andreas Hofmann; Samuel Clokie

Opis

A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Recenzje

'After seven editions between 1975 and 2010 this essential textbook has undergone a major update. The new editors, Drs Hofmann and Clokie, recruited many experts to revise individual chapters and added a significant amount of new material, thus making the book even more valuable. This eighth edition is basically a new book that covers the techniques of biochemistry and molecular biology in a very comprehensive manner - it does not go into great detail, but gives the reader a good introduction to all of them. Each chapter contains suggestions for further reading for those in need of a follow-up. Excellent diagrams and illustrations provide help in comprehension of the presented material. Since this text is largely aimed at undergraduate students, it fulfils its role extremely well.' Alexander Wlodawer, National Cancer Institute at Frederick

Spis treści

Foreword Keith Wilson and John Walker; Preface Samuel Clokie and Andreas Hofmann; Tables and resources Andreas Hofmann; 1. Life science studies Samuel Clokie and Andreas Hofmann; 2. Basic principles Parisa Amani and Andreas Hofmann; 3. Cell culture techniques Anwar Baydoun; 4. Recombinant DNA techniques and molecular cloning Ralph Rapley; 5. Preparative biochemistry Samuel Clokie; 6. Electrophoresis Ralph Rapley; 7. Immunochemical techniques Katja Fischer; 8. Flow cytometry John Grainger and Joanne Konkel; 9. Radioisotope techniques Robert Slater; 10. Clinical biochemistry Gillian Rumsby; 11. Microscopy Stephen Paddock; 12. Centrifugation Kay Ohlendieck and Stephen Harding; 13. Spectroscopic techniques Anne Simon and Andreas Hofmann; 14. Basic techniques probing molecular structure and interactions Anne Simon and Joanne Macdonald; 15. Mass spectrometric techniques Sonja Hess and James MacRae; 16. Fundamentals of bioinformatics Cinzia Cantacessi and Anna Protasio; 17. Fundamentals of cheminformatics Paul Taylor; 18. The Python programming language Timothy Stevens; 19. Processing and visualisation of experimental data Jean-Baptiste Cazier; 20. Fundamental genome sequencing and annotation Pasi Korhonen and Robin Gasser; 21. Fundamentals of proteomics Sonja Hess and Michael Weiss; 22. Fundamentals of metabolomics James MacRae; 23. Enzymes and receptors Megan Cross and Andreas Hofmann; 24. Drug discovery and development David Camp.

O autorze

Hofmann, Andreas Andreas Hofmann is Associate Professor at the Structural Chemistry Program Leader at Griffith University's Eskitis Institute and an Honorary Senior Research Fellow at the Faculty of Veterinary and Agricultural Sciences at the University of Melbourne. He is also fellow of the Higher Education Academy (UK). Hofmann's research lab focusses on the structure and function of proteins in infectious and neurodegenerative diseases, and also develops computational tools. He is the author of Methods of Molecular Analysis in the Life Sciences (Cambridge, 2014) and Essential Physical Chemistry (2017). Clokie, Samuel Samuel Clokie is the principal bioinformatician at the Birmingham Women's and Children's Hospital, where he leads the implementation of bioinformatic techniques to decipher next generation sequencing data in clinical diagnostics. He is also an Honorary Senior Research Fellow at the Institute of Cancer and Genomic Sciences at the University of Birmingham and before that was a Research Fellow at the National Institutes of Health in Bethesda.