Virus-Induced Gene Silencing: Methods and Protocols

ISBN-13: 9781627032773 / Angielski / Twarda / 2013 / 221 str.

Annette Becker

Opis

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant's development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.

Spis treści

1. Virus-Induced Gene Silencing (VIGS) in Plants – An Overview of Target Species and the Virus-Derived Vector Systems
                Matthias Lange, Aravinda L. Yellina, Svetlana Orashakova, and Annette Becker

 

2. Rationale for Developing New Virus Vectors to Analyze Gene Function in Grasses Through Virus-Induced Gene Silencing
                Hema Ramanna, Xin Shun Ding, and Richard S. Nelson

 

3. Virus Induced Gene Silencing for Rice Using Agroinoculation

                Arunima Purkayastha, Shweta Sharma, and Indranil Dasgupta

 

4. Utilizing Virus Induced Gene Silencing for the Functional Characterization of Maize Genes During Infection with the Fungal Pathogen Ustilago maydis
                Karina van der Linde and Gunther Doehlemann

 

5. Analysis of Developmental Control Genes Using Virus Induced Gene Silencing
                Koen Geuten, Tom Viaene, Dries Vekemans, Sofia Kourmpetli, and Sinead Drea

 

6. Virus Induced Gene Silencing in the Rapid Cycling Columbine Aquilegia coerulea 'Origami'
                Bharti Sharma and Elena M. Kramer

 

7. Virus-Induced Gene Silencing of the Alkaloid-Producing Basal Eudicot Model Plant Eschscholzia californica (California Poppy)
                Dawit G. Tekleyohans, Sabrina Lange, and Annette Becker

 

8. Virus-Induced Gene Silencing Using Artificial miRNAs in Nicotiana benthamiana
                Yang Tang, Yizhen Lai, and Yule Liu

 

9. The Use of VIGS Technology to Study Plant-Herbivore Interactions

                Ivan Galis, Meredith C. Schuman, Klaus Gase, Christian Hettenhausen, Markus Hartl, Son T. Dinh, Jianqiang Wu, Gustavo Bonaventure, and Ian T. Baldwin

 

10. Virus-Aided Gene Expression and Silencing Using TRV for Functional Analysis of Floral Scent-Related Genes
                Ben Spitzer-Rimon, Alon Cna’ani, and Alexander Vainstein

 

11. Virus-Induced Gene Silencing in Soybean and Common Bean

                Chunquan Zhang, Steven A. Whitham, and John H. Hill

 

12. Functional Genomic Analysis of Cotton Genes with Agrobacterium-Mediated Virus-Induced Gene Silencing
                Xiquan Gao and Libo Shan

 

13. Highly Efficient Virus-Induced Gene Silencing in Apple and Soybean by Apple latent spherical virus vector and Biolistic Inoculation

                Noriko Yamagishi and Nobuyuki Yoshikawa

 

14. VIGS A Tool to Study Fruit Development in Solanum lycopersicum

Josefina-Patricia Fernandez-Moreno, Diego Orzaez, and Antonio Granell

 

15. A Protocol for VIGS in Arabidopsis thaliana Using a "One-Step" TYMV-Derived Vector                Isabelle Jupin

 

16. Virus-Induced Gene Silencing in Strawberry Fruit

                Haifeng Jia and Yuanyue Shen

O autorze

Annette Becker, Dr. phil., Studium der Kunstgeschichte, Archäologie, Philosophie und Betriebswirtschaftslehre in Mainz, München und Rom. Kuratorin am Deutschen Architekturmuseum

Blurb

Plants are amazing organisms to study, some are important sources for pharmaceuticals, and others can help to elucidate molecular mechanisms required for a plant’s development and its interactions with the biotic or abiotic environment. Functional genomics is vastly lagging behind the speed of genome sequencing as high-throughput gene function assays are difficult to design, specifically for non-model plants. Bioinformatics tools are useful for gene identification and annotation but are of limited value for predictions concerning gene functions as gene functions are uncovered best by experimental approaches. Virus-Induced-Gene-Silencing (VIGS) is an easy to use, fast, and reliable method to achieve down regulation of target gene expression. Virus-Induced Gene Silencing: Methods and Protocols provides detailed protocols for VIGS experiments in several plant species including model and non-model plants. Also included in this book are recently developed protocols for VIGS-derived microRNA production in the plant or protein over expression, as well as chapters devoted to summarizing the molecular mechanisms of VIGS action and the vector systems developed so far. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Virus-Induced Gene Silencing: Methods and Protocols serves as a valuable resource for researchers from diverse fields of plant biology interested in experimental approaches to analyzing gene functions.