Understanding Light Microscopy

ISBN-13: 9780470973752 / Angielski / Twarda / 2019 / 848 str.

Jeremy Sanderson

Opis

This textbook introduces readers to the modern light microscope. It can either be used alone or in conjunction with a practical course. The book begins with an account of how the light microscope works, and how to set up the instrument for optimum performance.

Spis treści

About the Author ix Acknowledgements xi Look-Up Guide to Feature Boxes by Theme xii Glossary and Definitions xiv Notes xxiv Introduction xxvii 1 Our Eyes and the Microscope 1 2 Light 29 3 Basic Microscope Optics 55 4 Microscope Anatomy and Design 75 5 Ergonomics 91 6 Optical Aberrations of the Microscope 101 7 The Microscope Objective 127 8 Condensers and Eyepieces 161 9 Illumination in the Microscope 177 10 Diffraction and Image Formation in Microscopy 211 11 Contrast Generation and Enhancement 243 12 Reflected-Light Microscopy 289 13 Polarised-Light Microscopy: Part 1 - Theory 317 14 Polarised-Light Microscopy: Part 2 - Applied 347 15 Fluorescence Microscopy 383 16 Fluorophores and Fluorescent Proteins 405 17 Optical Sectioning and Confocal Microscopy 425 18 Operating the Confocal Microscope 447 19 Light-Sheet Microscopy 483 20 Bleed-Through and Spectral Unmixing 507 21 Deconvolution 523 22 Multi-Photon Microscopy 543 23 Total Internal Reflection Fluorescence Microscopy 561 24 FRAP and FRET 569 25 Colocalisation 587 26 Super-Resolution Fluorescence Microscopy 613 27 Choosing a Microscope Platform and Core Imaging Facilities 637 28 Biological Specimen Preparation 663 29 Materials Specimen Preparation 687 30 Recording the Image: Part 1 - Theory 707 31 Recording the Image: Part 2 - Applied 733 Appendices 1 Buying, and Tendering for, a Light Microscope 769 2 Troubleshooting Poor Image Quality 773 3 The Michel-Lévy Interference Colour Chart 775 4 Cleaning and Maintenance of the Light Microscope 779 5 Selected Suppliers 783 6 Historical Background 787 7 Timeline of Key Events 791 Index 799

O autorze

JEREMY SANDERSON is a Bio-Imaging specialist at the Mammalian Genetics Unit, MRC Harwell Institute, in the UK. He is Chartered Scientist and a Fellow of the Royal Microscopical Society and has served both on the Executive Council and the LM Section Committee.