Unconventional Protein Secretion: Methods and Protocols

ISBN-13: 9781493981441 / Angielski / Miękka / 2018 / 273 str.

Pompa, Andrea

Opis

This volume presents relevantbackground information to understanding the molecular basis governingunconventional protein secretion (UPS), and in particular explores the latesttechniques and protocols that have been successfully applied for the study ofthis topic. Detailed chapters include an overview of conventional andunconventional secretory pathways along with multidisciplinary approaches andmethods used for UPS analysis in different organisms. Written in the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessary materials and reagents, step-by-step,readily reproducible laboratory protocols, and tips on troubleshooting andavoiding known pitfalls.Authoritative and cutting-edge, UnconventionalProtein Secretion: Methods and Protocols will be usefulfor all interested in the secretory pathway field as well as applications incell biology, cell development, biomedical research, and healthcare.

Spis treści

Part I: Background

1. ER to Golgi Dependent Protein Secretion: The Conventional Pathway

Corrado Viotti

2. Unconventional Protein Secretion in Animal Cells

Fanny Ng and Bor Luen Tang

3. Unconventional Protein Secretion in Plants

Destiny J. Davis, Byung-Ho Kang, Angelo  S. Heringer, Thomas  E. Wilkop, and Georgia Drakakaki

Part II: UPS Contemplates Multidisciplinary Approaches for Plants

4. Chemical Secretory Pathway Modulation in Plant Protoplasts

Francesca De Marchis, Andrea Pompa, and Michele Bellucci

5. From Cytosol to the Apoplast: The Hygromycin Phosphotransferase (HYG^R) Model in Arabidopsis

Haiyan Zhang and Jinjin Li

6. Following the Time-Course of Post-Pollination Events by Transmission Electron Microscopy (TEM): Buildup of Exosome-Like Structures with Compatible Pollinations

Darya Safavian, Jennifer Doucet, and Daphne R.  Goring

Part III: UPS Contemplates Multidisciplinary Approaches for Animals and Fungi

7. Investigating Alternative Transport of Integral Plasma Membrane Proteins from the ER to the Golgi: Lessons from the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR)

Margarida D. Amaral, Carlos M. Farinha, Paulo Matos, and Hugo M. Botelho

8. Quantification of a Non-Conventional Protein Secretion: The Low Molecular Weight FGF-2 Example

Tania Arcondéguy, Christian Touriol, and Eric Lacazette

9. Human Primary Keratinocytes as a Tool for the Analysis of Caspase-1-Dependent Unconventional Protein Secretion

Gerhard E. Strittmatter, Martha Garstkiewicz, Jennifer Sand, Serena Grossi, Hans-Dietmar Beer

10. A Reporter System to Study Unconventional Secretion of Proteins Avoiding N-glycosylation in Ustilago maydis

Janpeter Stock, Marius Terfrüchte, and Kerstin Schipper

11. Stress-inducible Protein 1 (STI1): Extracellular Vesicles Analysis And Quantification

Marcos Vinicios Salles Dias, Vilma Regina Martins, and Glaucia Noeli Maroso Hajj

12. Analysis of Yeast Extracellular Vesicles

Marcio L. Rodrigues, Debora L. Oliveira, Gabriele Vargas, Wendell Girard-Dias, Anderson J. Franzen, Susana Frasés, Kildare Miranda, and Leonardo Nimrichter

13. Exploring the Leishmania Hydrophilic Acylated Surface Protein B (HASPB) Export Pathway by Live Cell Imaging Methods

Lorna MacLean, Helen Price, and Peter O’Toole

14. Characterization of the Unconventional Secretion of the Ebola Matrix Protein VP40

Olivier Reynard and Mathieu Mateo

15. Role and Characterization of synuclein-γ Unconventional Protein Secretion in Cancer Cells

Caiyun Liu, Like Qu, and Chengchao Shou

Part IV: Secretome Isolation from Plant and Animal Samples to Identify Leaderless Secretory Proteins (LSP)

16. Characterization of the Tumor Secretome from Tumor Interstitial Fluid (TIF)

Pavel Gromov and Irina Gromova

17. Vacuum Infiltration-Centrifugation Method for Apoplastic Protein Extraction in Grapevine

Bertrand Delaunois, Fabienne Baillieul, Christophe Clément, Philippe Jeandet, and Sylvain Cordelier

18. Isolation of Exosome-Like Vesicles from Plants by Ultracentrifugation on Sucrose/Deuterium Oxide (D₂O) Density Cushions

Christopher Stanly, Immacolata Fiume, Giovambattista Capasso, and Gabriella Pocsfalvi