Two-Hybrid Systems: Methods and Protocols

ISBN-13: 9781493993109 / Angielski / Miękka / 2019 / 355 str.

Luis Onate-Sanchez

Spis treści

1. Protein Interactomics by Two-Hybrid Methods

            Soon Gang Choi, Aaron Richardson, Luke Lambourne, David E. Hill, and Marc Vidal

 

Part I: Yeast Hybrid Systems to Study Protein, Nucleic Acid, and Ligand Interactions

 

2. Making the Right Choice: Critical Parameters of the Y2H Systems

            Jitender Mehla, J. Harry Caufield, and Peter Uetz

 

3. Identification of Protein-Protein Interactions Using Pool-Array-Based Yeast Two-Hybrid Screening

            Thomas Lathouwers, Jeroen Wagemans, and Rob Lavigne

 

4. Detecting Interactions of Membrane Proteins: The Split-Ubiquitin System

            Lisa Yasmin Asseck and Christopher Grefen

 

5. The Ras Recruitment System (RRS) for the Identification and Characterization of Protein-Protein Interactions

            Ami Aronheim

 

6. A Bacterial Adenylate Cyclase-Based Two-Hybrid System Compatible with Gateway® Cloning

            Macy G. Olson, Megan Goldammer, Emilie Gauliard, Daniel Ladant, and Scot P. Ouellette

 

7. Analyses of Protein Interaction Networks Using Computational Tools

            Shaowei Dong and Nicholas J. Provart

 

8. Options and Considerations When Using a Yeast One-Hybrid System

            Jared A. Sewell and Juan I. Fuxman Bass

 

9. Screening Arrayed Libraries with DNA and Protein Baits to Identify Interacting Proteins

            Rocío Sánchez-Montesino and Luis Oñate-Sánchez

 

10. Construction of Arabidopsis Transcription Factor ORFeome Collections and Identification of Protein-DNA Interactions by High-Throughput Yeast One-Hybrid Screens

            S. Earl Kang, Ghislain Breton, and Jose L. Pruneda-Paz

 

11. Transcription Factor-Centered Yeast One-Hybrid Assay

            Xiaoyu Ji, Liuqiang Wang, Dandan Zang, and Yucheng Wang

 

12. The Yeast Three-Hybrid System for Protein Interactions

            Franziska Glass and Mizuki Takenaka

 

13. The Yeast Three-Hybrid System for Screening RNA-Binding Proteins in Plants

            Sung Ki Cho and David J. Hannapel

 

14. Using Yeast Hybrid System to Identify Proteins Binding to Small Molecules

            You Wang, David S. Letham, Peter C.L. John, and Ren Zhang

 

15. Shuttling of Entire Libraries from an Entry Vector to a Destination Vector of the Gateway System

            Alexander Heyl

 

Part II: Experimental Approaches to Study Protein-Protein and Protein-DNA Interactions in Plants and Human Cells

 

16. Bimolecular Fluorescence Complementation with Improved Gateway-Compatible Vectors to Visualize Protein-Protein Interactions in Plant Cells

            Shino Goto-Yamada, Kazumi Hikino, Mikio Nishimura, Tsuyoshi Nakagawa, and Shoji Mano

 

17. Bimolecular Fluorescence Complementation to Visualize Protein-Protein Interactions in Human Cells Based on Gateway Cloning Technology

            Adriana Lepur and Oliver Vugrek

 

18. KISS: A Mammalian Two-Hybrid Method for In Situ Analysis of Protein-Protein Interactions

            Delphine Masschaele, Sarah Gerlo, Irma Lemmens, Sam Lievens, and Jan Tavernier

 

19. Coimmunoprecipitation of Interacting Proteins in Plants

            Alfonso Muñoz and M. Mar Castellano

 

20. Analysis of Protein-Protein Interaction by Co-IP in Human Cells

            Zhenyuan Tang and Yoshinori Takahashi

 

21. Tandem Affinity Purification of Protein Complexes from Arabidopsis Cell Cultures

            Marta García-León, Elisa Iniesto, and Vicente Rubio

 

22. Transient Transactivation Studies in Nicotiana benthamiana Leaves

            Pilar Lasierra and Salomé Prat

 

23. The Use of the Chromatin Immunoprecipitation Technique for In Vivo Identification of Plant Protein-DNA Interactions

            José A. Jarillo, Dorota N. Komar, and Manuel Piñeiro

 

24. Chromatin Immunoprecipitation for Identification of Protein-DNA Interactions in Human Cells

            Bjørk Ditlev Larsen, Martin Rønn Madsen, Ronni Nielsen, and Susanne Mandrup

Blurb

This detailed book explores the numerous applications of the yeast two-hybrid (Y2H) system, one of the most commonly used methods to detect protein-protein interactions. Beginning with an overview, the volume then continues by elucidating different methodologies to detect protein-protein interactions in yeast nucleus, membrane, cytoplasm, and bacteria, computational analyses of protein interaction networks, technical variations in yeast one-hybrid (Y1H) systems together with classical and more elaborated yeast methods to detect protein-DNA interactions, as well as protocols to analyze ternary protein interactions and RNA-protein and ligand-protein interactions.  The book concludes with a section on methods in plant and mammalian cells that can be used to identify novel protein-protein and protein-DNA interactions and test (qualitatively and/or quantitatively) those observed in yeast. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab. 

Authoritative and practical, Two-Hybrid Systems: Methods and Protocols serves as an ideal guide to the uses of this exceedingly valuable technique.