Trichoderma Reesei: Methods and Protocols

ISBN-13: 9781071610473 / Angielski / Twarda / 2020 / 364 str.

Astrid R. Mach-Aigner; Roland Martzy

Spis treści

1. Ecological Genomics and Evolution of Trichoderma reesei

Komal Chenthamara, Irina S. Druzhinina, Mohammad J. Rahimi, Marica Grujic, and Feng Cai

 

2. Industrial Relevance of Trichoderma reesei as an Enzyme Producer

            Amanda J. Fischer, Suchindra Maiyuran, and Debbie S. Yaver

 

3. The Potential of Synthetic Biology for Trichoderma reesei

            Roland Martzy and Astrid R. Mach-Aigner

 

4. Resistance Marker- and Gene Gun-Mediated Transformation of Trichoderma reesei

            Monika Schmoll and Susanne Zeilinger

 

5. Use of Auxotrophic Markers for Targeted Gene Insertions in Trichoderma reesei

            Irene Tomico-Cuenca and Christian Derntl

 

6. Open the Pores: Electroporation for the Transformation of Trichoderma reesei

            Franziska Wanka

 

7. Sexual Crossing of Trichoderma reesei

            Rita B. Linke

 

8. CRISPR/Cas9-Mediated Genome Editing of Trichoderma reesei

            Gen Zou and Zhihua Zhou

 

9. The Copper-Controlled RNA Interference System in Trichoderma reesei

            Lei Wang, Weixin Zhang, Xiangfeng Meng, and Weifeng Liu

 

10. Batch Cultivation of Trichoderma reesei

            Birgit Jovanović

 

11. Image Analysis Method for the Characterization of Trichoderma reesei during Fermentations

            Nicolas Hardy, Maxime Moreaud, and Fadhel Ben Chaabane

 

12. Measurement of Cellulase and Xylanase Activities in Trichoderma reesei

            Qing-Shan Meng, Fei Zhang, Chen-Guang Liu, Feng-Wu Bai, and Xin-Qing Zhao

 

13. Flow Cytometry for Filamentous Fungi

            Matthias G. Steiger

 

14. Molecular Identification of Trichoderma reesei

            Mohammad Rahimi, Feng Cai, Marica Grujic, Komal Chenthamara, and Irina S. Druzhinina

 

15. In Vivo Footprinting Analysis in Trichoderma reesei

            Alice Rassinger

 

16. RNA Characterization in Trichoderma reesei

            Petra Till

 

17. Proteomic Profiling of the Secretome of Trichoderma reesei

            SoFong Cam Ngan and Siu Kwan Sze

 

18. Transcriptomics in Trichoderma reesei

            Amanda C.C. Antonieto and Roberto N. Silva

 

19. The Comprehensive and Reliable Detection of Secondary Metabolites in Trichoderma reesei: A Tool for the Discovery of Novel Substances

            Bernhard Seidl, Christoph Bueschl, and Rainer Schuhmacher

 

20. In Silico Gene Analysis and Oligonucleotide Design for Construction of Expression Vectors

            Bernhard Seiboth

 

21. PacBio Long-Read Sequencing, Assembly, and Funannotate Reannotation of the Complete Genome of Trichoderma reesei QM6a

            Wan-Chen Li and Ting-Fang Wang

 

22. TSETA: A Third-Generation Sequencing-Based Computational Tool for Mapping and Visualization of SNPs, Meiotic Recombination Products, and RIP Mutations

            Hou-Cheng Liu, Wan-Chen Li, and Ting-Fang Wang

Blurb

This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Trichoderma reesei: Methods and Protocols serves as an ideal guide for a wide range of audiences, from students who want to familiarize themselves with basic research protocols to experienced scientists who are planning to establish a new method in their laboratories, working with this dynamic fungus.