Transforming Growth Factor-Beta Protocols

ISBN-13: 9781489943989 / Angielski / Miękka / 2013 / 165 str.

Philip H. Howe

Opis

Philip H. Howe and a group of well-versed experimentalists present the first major volume a collection of indispensable classic and cutting-edge TGF assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGF to methods for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature.
Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGF induces and modulates physiological behavior.

Recenzje

"This book would be most useful to a lab researcher who had not previously been studying TGF-ß. Having all the practical protocols for preparing, assaying, and studying this important molecule in a small volume could be very useful for such a researcher. "-Doody's Health Science Book Review Journal

Spis treści

In Vitro Assays for Measuring TGFb Growth Stimulation and Inhibition, M. Edens and E. B. Leof. Measurements of Active TGFb Generated by Culture Cells, R. Mazzieri, J. S. Munger, and D. B. Rifkin. Methods for Iodination of Active TGFb, TGFb Receptor Crosslinking and Immunoprecipitation of TGFb-Receptor Complexes, D. Danielpour. Detection of TGFb in Body Fluids and Tissues, A. H. Limper. Analysis of TGFb-Mediated Synthesis of Extracellular Matrix Components, B. A. Hocevar and P. H. Howe. Characterization of SMAD Phosphorylation and SMAD/Receptor Interaction, A. Mehra, L. Attisano, and J. L. Wrana. Promoter Analysis of TGFb-Responsive Genes by Transient Transfection and Deletional/Mutational Analysis, G. Jin and P. H. Howe. Regulation of AP-1 Activity by TGFb, B. A. Hocevar and P. H. Howe. CDK Pathway: Cyclin-Dependent Kinases (cdks) and Cyclin-Dependent Kinase Inhibitors, D. M. Gitig and A. Koff. Activation of the Mitogen-Activated Protein Kinase Pathway by TGFb, J. Yue and S. Markowitz. Detection of TGFb Type II Receptor Hotspot Mutations: The BAT-RII Assay, L. L. Myeroff and S. Markowitz. Mutation Detection in the TGFb Receptors and SMAD Genes: RT-PCR and Sequencing, L. L. Myeroff, H. He, S. P. Fink, and S. Markowitz. Analysis of TGFb-Inducible Apoptosis, T. L. Brown, S. Patil, and P. H. Howe. Index.

Blurb

The recent isolation and identification of the TGFß receptors, as well as their intracellular mediating proteins, has driven the development of many new methodologies and techniques for the study of TGFß. In Transforming Growth Factor-Beta Protocols, Philip H. Howe and a group of well-versed experimentalists present the first major collection of indispensable classic and cutting-edge TGFß assays. Described in great detail to ensure robust and successful results, these readily reproducible techniques range from the growth inhibition assay for TGFß to methodologies for monitoring its interactions with the mediating proteins. Extensive notes discuss potential pitfalls and provide tips on how to avoid failures, and throughout, emphasis is given to detailing those technical steps critical for experimental success that are often omitted in the primary literature.
Concise and highly practical, Transforming Growth Factor-Beta Protocols provides today's molecular and cell biologists-both expert and novice-with time-tested methods for the identification and analysis of the signal transduction pathways by which TGFß induces and modulates physiological behavior.