Tissue-Protective Cytokines: Methods and Protocols

ISBN-13: 9781627033077 / Angielski / Twarda / 2013 / 328 str.

Pietro Ghezzi

Opis

While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines. The prototypic tissue-protective cytokine is EPO. Initially described as neuro-protective, it is beneficial in animal models of ischemic and other types of injury. Scientists had to overcome the notion that EPO had only erythropoietic actions, was only produced by the kidney, and that its receptor was only present in erythroid progenitor cells. The use of in vitro and in vivo disease models was essential to demonstrate the protective effects of EPO. Reproducible models will be needed for the further study of the mechanism of action of EPO and for the identification of other tissue-protective cytokines. In Tissue-Protective Cytokines: Methods and Protocols, expert researchers in the field detail the key models that have been used to characterize the tissue-protective actions of cytokines. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, provide step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Tissue Protective Cytokines: Methods and Protocols aids scientists in continuing to study tissue-protection that will be a new field of interest of cytokine biology, both in discovering novel actions of known cytokines and in developing new drugs.

Spis treści

1. Erythropoietin and Engineered Innate Repair Activators

Michael Brines  and Anthony Cerami 

 

2. Epo and Non-hematopoietic Cells: What Do We Know?

Omolara O. Ogunshola and Anna Yu. Bogdanova

 

3. Tissue Protective Cytokines: Structure and Evolution

Pietro Ghezzi  and Darrell Conklin

 

4. The Regenerative Activity of Interleukin-6

Eithan Galun^, and Stefan Rose-John

 5. Brain Ischemic Injury in Rodents: The Protective Effect of EPO

Annelise Letourneur, Edwige Petit, Simon Rousse, Omar Touzani , and Myriam Bernaudin

 

6. Experımental Traumatıc Spınal Cord Injury

Zubeyde Erbayraktar, Necati Gokmen, Osman Yilmaz, Serhat Erbayraktar,

 

7. Erythropoietin as a Neuroprotectant for Neonatal Brain Injury: Animal Models

Christopher M. Traudt  and Sandra E. Juul

 

8. Evaluating Effects of EPO in Rodent Behavioral Assays Related to Depression

Catharine H. Duman and Samuel S. Newton

 9. Erythropoietin and Cytoprotective Cytokines in Experimental Traumatic Brain Injury

Samson Kumar Gaddam, Jovany Cruz, Claudia Robertson

 

10. Therapeutic Efficacy of Erythropoietin in Experimental Autoimmune Encephalomyelitis in Mice, a Model of Multiple Sclerosis

Ilaria Cervellini, Pietro Ghezzi, Manuela Mengozzi

 

11. Deciphering the Intracellular Signaling of Erythropoietin in Neuronal Cells

Murat Digicaylioglu

 

12. Assessment of Allodynia Relief by Tissue-protective Molecules in a Rat Model of Nerve Injury-induced Neuropathic Pain

Maarten Swartjes, Marieke Niesters, Albert Dahan

 

13. Intra-Epidermal Nerve Fibres Density and Nociception in EPO-treated Type 1 Diabetic Rats with Peripheral Neuropathy

Bianchi Roberto, Lombardi Raffaella, Porretta-Serapiglia Carla, Lauria Giuseppe

 

14. ARA290 in a Rat Model of Inflammatory Pain

Dr Andrew Dilley  

15. In Vivo Angiogenic Activity of Erythropoietin

Domenico Ribatti

 

16. Photoreceptor Degeneration in Mice: Adeno-associated Viral (AAV) Vector-Mediated Delivery of Erythropoietin

P. Colella and A. Auricchio

 

17. Myocardial Infarction - Cardioprotection by Erythropoietin

Mark I. Talan and Roberto Latini

 

18. Using Plethysmography to Determine Erythropoietin’s Impact on Neural Control of Ventilation

Tommy Seaborn, Max Gassmann and Jorge Soliz ^{19. Cerebral Malaria: Protection by Erythropoietin}

 Anne-Lise Bienvenu and Stephane Picot

 

18. Using Plethysmography to Determine Erythropoietin’s Impact on Neural Control of Ventilation

Tommy Seaborn, Max Gassmann and Jorge Soliz ^{19. Cerebral Malaria: Protection by Erythropoietin}

 Anne-Lise Bienvenu and Stephane Picot

 19. Cerebral Malaria: Protection by Erythropoietin

 Anne-Lise Bienvenu and Stephane Picot

Omolara O. Ogunshola and Anna Yu. Bogdanova

 

3. Tissue Protective Cytokines: Structure and Evolution

Pietro Ghezzi  and Darrell Conklin

 

4. The Regenerative Activity of Interleukin-6

Eithan Galun^, and Stefan Rose-John

 5. Brain Ischemic Injury in Rodents: The Protective Effect of EPO

Annelise Letourneur, Edwige Petit, Simon Rousse, Omar Touzani , and Myriam Bernaudin

 

6. Experımental Traumatıc Spınal Cord Injury

Zubeyde Erbayraktar, Necati Gokmen, Osman Yilmaz, Serhat Erbayraktar,

 

7. Erythropoietin as a Neuroprotectant for Neonatal Brain Injury: Animal Models

Christopher M. Traudt  and Sandra E. Juul

 

8. Evaluating Effects of EPO in Rodent Behavioral Assays Related to Depression

Catharine H. Duman and Samuel S. Newton

 9. Erythropoietin and Cytoprotective Cytokines in Experimental Traumatic Brain Injury

Samson Kumar Gaddam, Jovany Cruz, Claudia Robertson

 

10. Therapeutic Efficacy of Erythropoietin in Experimental Autoimmune Encephalomyelitis in Mice, a Model of Multiple Sclerosis

Ilaria Cervellini, Pietro Ghezzi, Manuela Mengozzi

 

11. Deciphering the Intracellular Signaling of Erythropoietin in Neuronal Cells

Murat Digicaylioglu

 

12. Assessment of Allodynia Relief by Tissue-protective Molecules in a Rat Model of Nerve Injury-induced Neuropathic Pain

Maarten Swartjes, Marieke Niesters, Albert Dahan

 

13. Intra-Epidermal Nerve Fibres Density and Nociception in EPO-treated Type 1 Diabetic Rats with Peripheral Neuropathy

Bianchi Roberto, Lombardi Raffaella, Porretta-Serapiglia Carla, Lauria Giuseppe

 

14. ARA290 in a Rat Model of Inflammatory Pain

Dr Andrew Dilley  

15. In Vivo Angiogenic Activity of Erythropoietin

Domenico Ribatti

 

16. Photoreceptor Degeneration in Mice: Adeno-associated Viral (AAV) Vector-Mediated Delivery of Erythropoietin

P. Colella and A. Auricchio

 

17. Myocardial Infarction - Cardioprotection by Erythropoietin

Mark I. Talan and Roberto Latini

 

18. Using Plethysmography to Determine Erythropoietin’s Impact on Neural Control of Ventilation

Tommy Seaborn, Max Gassmann and Jorge Soliz ^{19. Cerebral Malaria: Protection by Erythropoietin}

 Anne-Lise Bienvenu and Stephane Picot

 

18. Using Plethysmography to Determine Erythropoietin’s Impact on Neural Control of Ventilation

Tommy Seaborn, Max Gassmann and Jorge Soliz ^{19. Cerebral Malaria: Protection by Erythropoietin}

 Anne-Lise Bienvenu and Stephane Picot

 19. Cerebral Malaria: Protection by Erythropoietin

 Anne-Lise Bienvenu and Stephane Picot

Blurb

While many cytokines are known for their inflammatory action, there is a growing interest in the tissue-protective effects of some cytokines. The prototypic tissue-protective cytokine is EPO. Initially described as neuro-protective, it is beneficial in animal models of ischemic and other types of injury. Scientists had to overcome the notion that EPO had only erythropoietic actions, was only produced by the kidney, and that its receptor was only present in erythroid progenitor cells. The use of in vitro and in vivo disease models was essential to demonstrate the protective effects of EPO. Reproducible models will be needed for the further study of the mechanism of action of EPO and for the identification of other tissue-protective cytokines.

 In Tissue-Protective Cytokines: Methods and Protocols, expert researchers in the field detail the key models that have been used to characterize the tissue-protective actions of cytokines. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, provide step-by-step laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 Thorough and intuitive, Tissue Protective Cytokines: Methods and Protocols aids scientists in continuing to study tissue-protection that will be a new field of interest of cytokine biology, both in discovering novel actions of known cytokines and in developing new drugs.