Time-alignment in HPLC-MS

ISBN-13: 9783659130205 / Angielski / Miękka / 2012 / 108 str.

Jan Urban; Pavel Hrouzek; Dalibor Tys

Opis

We present time alignment based on blank measurement as analytical approach for reduction of time shift in high performance liquid chromatography - mass spectrometry (HPLC-MS). Our approach is based on the measurement and comparison of the distinct features of the blank and the analyzed sample. Blank measurement based time alignment (BBTA) utilizes solvent contaminants and other important events detectable both in blank run and the compared experiment for alignment of multiple 2D chromatograms. Several metrics for comparison of mass spectra were evaluated. Marker selection works efficiently even when no peaks are detected. It is capable of analyzing the baseline contribution in analyte measurement when the knowledge of blank behavior is limited. Finally, BBTA because of its simplicity has the potential to be widely used in HPLC-MS data treatment.

O autorze

Dr. Jan Urban (South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses) has obtained his PhD in Biophysics in 2010. Together with assoc. prof. Dalibor tys, and dr. Pavel Hrouzek (Institute of Microbiology AS CR), they colaborate in the subject of mass spectrometry since 2005.