Thyroid Hormone Receptors: Methods and Protocols

ISBN-13: 9781489938794 / Angielski / Miękka / 2013 / 223 str.

Aria Baniahmad

Opis

A panel of outstanding investigators surveys and explains the major cutting-edge methods used in thryroid receptor (TR) research and explains their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TR in development and knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and in living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for studying the oncogene v-Erba in blood cell differentiation, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver.

Spis treści

Introduction to Thyroid Hormone Receptors Aria Baniahmad Null Mutant Mice for Thyroid Hormone Receptors Karine Gauthier, Denise Aubert, Olivier Chassande, Frederic Flamant, and Jacques Samarut Transgenic Targeting of a Dominant Negative Corepressor to Liver and Analyses by cDNA Microarray Xu Feng, Paul Meltzer, and Paul M. Yen The Role of Thyroid Hormone Receptors in the Heart Wolfgang H. Dillmann and Bernd R. Gloss Analysis of Thyroid Hormone-Dependent Genes in the Brain by In Situ Hybridization Juan Bernal and Ana Guadaño-Ferraz. The v-erbA Oncogene: Assessing Its Differentiation-Blocking Ability Using Normal Chicken Erythrocytic Progenitor Cells, Olivier Gandrillon Gene Regulation by Thyroid Hormone Receptors Helmut Dotzlaw and Aria Baniahmad Molecular Analysis of Human Resistance to Thyroid Hormone Syndrome Sunnie M. Yoh and Martin L. Privalsky Function of Thyroid Hormone Receptors During Amphibian Development Sashko Damjanovski, Laurent M. Sachs, and Yun-Bo Shi Transcriptional Regulation by Thyroid Hormone Receptor in Chromatin Jiemin Wong Gene Activation by Thyroid Hormone Receptor In Vitro and Purification of the TRAP Coactivator Complex Joseph D. Fondell Index

Blurb

Because thyroid hormone receptors (TRs) are important regulators of a large number of biological processes-ranging from brain development to control of vision in vertebrates-abnormal functioning of these receptors can have serious medical consequences. In Thyroid Hormone Receptors: Methods and Protocols, a panel of outstanding investigators surveys and explains the major cutting-edge methods used in TR research and their practical experimental details. Described in step-by-step detail to ensure robust experimental results, the techniques presented cover a wide variety of key areas, including TRs in development and generation of knockout (mouse and Xenopus), transcriptional regulation by TRs in both cell-free systems and living cells, and TR mutant analysis of patients. Additional methods provide powerful tools for the isolation of TR-regulated protein complexes, for the study of blood cell differentiation through the oncogene v-erbA, and for target gene analysis in the brain. Microarray chip methods are also presented for analyzing the organs of transgenic mice to identify target genes in the liver. Each protocol has been developed by a hands-on expert and contains full explanatory notes, troubleshooting tips, and helpful comments on avoiding pitfalls.
Broad ranging and highly practical, Thyroid Hormone Receptors: Methods and Protocols offers endocrinologists, geneticists, biochemists, and research physicians a comprehensive collection of powerful methods useful not only for all major areas of TR biology and analysis, but also for nuclear hormone receptors in general.