Thrombin: Structure and Function

ISBN-13: 9781461364504 / Angielski / Miękka / 2012 / 438 str.

Lawrence J. Berliner; Lawrenglishce J. Berliner

Opis

Research on thrombin structure and function has progressed significantly over the past three decades. We are continually discovering new functions f()r this enzyme in biology. Yet, until quite recently, a full, detailed, three- dimensional picture of its structure was difficult to attain. We believe that this text represents a turning point and, more appropriately, a new start- ing point for thrombin studies. Our goal for this text is to present a thorough and rounded-out coverage of thrombin chemistry and biochem- istry in order to provide the biochemist and physiologist with an excellent desk reference on almost any thrombin-related problem. This volume is organized into three general thrombin topic areas: Structure, Biochemistry, and Physiology. In Part 1, Structure, we open with the complete three-dimensional x-ray structures of two inhibited human thrombin complexes, one of which is the thrombin-hirudin com- plex. These complexes are also addressed in the chapter on structural studies in solution, which include NMR, ESR, and fluorescence. Part 2, Biochemistry, includes chapters on synthetic thrombin inhibitors, protein inhibitors (e.g., antithrombin III, hirudin), and thrombin interactions with factor XIII. Part 3, Physiology, covers such topics as chemotactic activities, interactions with cell surfaces, and the vascular endothelium.

Spis treści

Structure: Nuclear Magnetic Resonance Studies of ThrombinFibrinopeptide and ThrombinHirudin Complexes (F. Ni et al.). ESR and Flourescence Studies of Thrombin Active Site Conformation (L.J. Berliner). Biochemistry: Synthetic Substrates and Inhibitors of Thrombin (J.C. Powers, C.M. Kam). Regulation of Thrombin by Antithrombin and Heparin Cofactor II (S.T. Olson, I. Björk). Hirudin Interactions with Thrombin (S.R. Stone, J.M. Maraganore). Physiology: Functional Domains in Thrombin Outside the Catalytic Site (R. BarShavit et al.). Post Clotting Cellular Effects of Thrombin Mediated by Interaction with HighAffinity Thrombin Receptors (D.H. Carney). Regulation of ThrombinInduced Endothelial Barrier Dysfunction and Prostaglandin Synthesis (J.G.N. Garcia et al.). 3 additional articles. Index.

O autorze

Dr. Lawrence J. Berliner is currently Professor and Chair of the Department of Chemistry and Biochemistry at the University of Denver after retiring from Ohio State University, where he spent a 32-year career in the area of biological magnetic resonance (EPR and NMR). He is the Series Editor for Biological Magnetic Resonance, which he launched in 1979.