Therapeutic Proteins

ISBN-13: 9781071634684 / Angielski / Twarda / 2023

Opis

This volume covers the latest key aspects of therapeutic protein applications. Chapters in this book cover topics such as the discovery, production, and conjugation of protein-proteins with discussions on the direction of future development and advancements; ways to use these engineering proteins for therapeutic and vaccine applications; and the use of modified protein nanocarriers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Therapeutic Proteins: Methods and Protocols is a valuable resource for any researcher who are interested in learning more about the field of therapeutic proteins. 

Spis treści

Preface…
Table of Contents…
Contributing Authors…

Part I Discovery, Production, and Conjugation of Therapeutic Proteins

1 Optimizing Cell-Free Protein Synthesis for Antimicrobial Protein Production
Tejaswini Ramakrishna Hegde, Ogechi Okocha Rufus, Joongoo Lee, and Seok Hoon Hong

2 Design and Construction of Antibody Fusion Proteins Incorporating Variable New Antigen Receptor (VNAR) Domains
Seth D. Ludwig, Angela Zhu, Ankith P. Maremanda, Helen M. Dooley, and Jamie B. Spangler

3 Selective and Site-Specific Incorporation of Non-Standard Amino Acids within Proteins for Therapeutic Applications
Neil D. Butler and Aditya M. Kunjapur

4 An Approach for Antigen-Agnostic Identification of Virus-Like Particle-Displayed Epitopes that Engage Specific Antibody V Gene Regions
Julianne Peabody, Susan Core, Larance Ronsard, Daniel Lingwood, David Peabody, and Bryce Chackerian

5 Split-Protein Therapeutic Platforms: Identifying Binder Pairs
Christine S. Nervig, James R. Gustat, and Shawn C. Owen

6 Metal-Mediated Ligand Affinity Chemistry (MLAC)  
Sailajah Gukathasan and Samuel G. Awuah

Part II Engineering Proteins for Therapeutic and Vaccine Applications

7 Recombinant Elastin‐Based Bioelastomers for Biomedical Applications
Paulina M. Babiak, Jason Minnich II, Jessica Torres, Sathvik Madduri, and Julie C. Liu

8 Engineering Hepatitis B Virus (HBV) Protein Particles for Therapeutic Delivery
Millicent O. Sullivan and Wilfred Chen

9 Design and Purification of Tag/Catcher AP205-Based Capsid Virus-Like Particle Vaccines
Kara-Lee Aves and Adam F. Sander

10 Protein Nanocarriers Capable of Encapsulating Both Hydrophobic and Hydrophilic Drugs
Jooyong Shin, Blair D. Cole, Moosavizadeh Seyedmohammad, Sung In Lim, and Yeongseon Jang

11 Unnatrual Amino Acid Engineering for Intracellular Delivery of Protein Therapeutics
Millicent O. Sullivan and Wilfred Chen

12 Formulation of Chitosan-Zein Nano-in-Microparticles for Oral DNA Delivery
Kari Heck, Eric Farris, and Angela K. Pannier

13 Engineering E2 Bionanoparticles for Targeted Delivery of Chemotherapeutics to Breast Cancer Cells
Millicent O. Sullivan and Wilfred Chen

14 Constructing Nucleic Acid Delivering Lipoproteoplexes from Coiled-Coil Supercharged Protein and Cationic Liposomes
Joseph Thomas, Jonathan Sun, and Jin Kim Montclare

15 Cancer Cell Silicification and Surface Functionalization to Create Microbial Mimetic Cancer Vaccines
Lorél Medina, Bryan Kaeh, and Rita E. Serda

16 Melt-Processing Virus-Like Particle-Based Vaccine Candidates into Biodegradable Polymer Implants
Armando A. Puente, Oscar A. Ortega-Rivera, David M. Wirth, Jonathan K. Pokorski, and Nicole F. Steinmetz

Subject Index List…