Therapeutic Oligonucleotides: Methods and Protocols

ISBN-13: 9781493958221 / Angielski / Miękka / 2016 / 340 str.

John Goodchild

Opis

This volume in the Methods in Molecular Biology(TM) series presents established and emerging methods for the application of oligonucleotides as therapeutics. Includes protocols for optimizing cell uptake, assaying gene knockdown, target site selection and more.

Recenzje

From the reviews:

"The book is written for both professionals and novices interested in the field of oligonucleotide therapeutics. It also would be appropriate for graduate students and research scientists interested in this area. ... This is an extremely useful resource that provides different ways that oligonucleotides may be applied as therapeutics. Very few books cover the field as thoroughly and as clearly as this one." (Omer Iqbal, Doody's Book Reviews, November, 2011)

Spis treści

1. Therapeutic Oligonucleotides

            John Goodchild

 

2. Dinucleotides Containing 3¢-S-Phosphorothiolate Linkages

            James W. Gaynor and Richard Cosstick

 

3. 2’-O,4’-C-Methyleneoxymethylene Bridged Nucleic Acids (2’,4’-BNACOC)

            Yoshiyuki Hari, Tetsuya Kodama, Takeshi Imanishi, and Satoshi Obika

 

4. A Non-covalent Peptide-based Strategy for Ex-vivo and In vivo Oligonucleotide Delivery

            Laurence Crombez, May C. Morris, Frederic Heitz, and Gilles Divita

 

5. Cell Penetrating Peptides-based Strategies for the Delivery of Splice Redirecting Antisense Oligonucleotides

            Samir El Andaloussi, Fatouma Said Hassane, Prisca Boisguerin, Rannar Sillard, Ulo Langel, and Bernard Lebleu

 

6. A Nanoparticle for Tumor Targeted Delivery of Oligomers

            Xinrong Liu, Yi Wang, and Donald J. Hnatowich

 

7. Light-directed Delivery of Nucleic Acids

            Sigurd Bøe, Lina Prasmickaite, Birgit Engesæter, and Eivind Hovig

 

8. Antibody Targeted siRNA Delivery

            Masoud M. Toloue and Lance P. Ford

 

9. Aptamer-Drug Conjugation for Targeted Tumor Cell Therapy

            Michael J. Donovan, Ling Meng, Tao Chen, Yunfei Zhang, Kwame Sefah, and Weihong Tan

 

10. Five-step Process for Screening Antisense Compounds for Efficacy: Gene Target IL-12Rb2

            Nikki B. Marshall, Laura L. Hauck, and Dan V. Mourich

 

11. Diverse Small Non-coding RNAs in RNA Interference Pathways

            Liande Li and Yi Liu

 

12. Quantification of siRNAs In vitro and In vivo

            Angie Cheng, Alexander V. Vlassov, and Susan Magdaleno

 

13. Optimization of Transfection Conditions and Analysis of siRNA Potency Using Real-time PCR

            Angie Cheng, Susan Magdaleno, and Alexander V. Vlassov

 

14. siRNA Knockdown of Gene Expression in Endothelial Cells

            Emily Dennstedt and Brad Bryan

15. Using RNA Interference in Schistosoma mansoni

            Rita Bhardwaj, Greice Krautz-Peterson, and Patrick J. Skelly

 

16. Performing the Labeled microRNA Pull-down (LAMP) Assay System: An Experimental Approach for High-throughput Identification of microRNA-target mRNAs

            Ren-Jun Hsu and Huai-Jen Tsai

 

17. Synthesis, Purification, and Characterization of Oligoribonucleotides that Act as Agonists of TLR7 and/or TLR8

            Tao Lan and Ekambar R. Kandimalla

 

18. Synthesis, Purification, and Characterization of Immune-Modulatory Oligodeoxynucleotides that Act as Agonists of Toll-like Receptor 9

            Mallikarjuna Reddy Putta, Dong Yu, and Ekambar R. Kandimalla

 

19. Surface Plasmon Resonance Investigation of RNA Aptamer-RNA Ligand Interactions

            Carmelo Di Primo, Eric Dausse, and Jean-Jacques Toulmé

 

20. Practical Considerations for Analyzing Antigene RNAs (agRNAs): RNA Immunoprecipitation of Argonaute Protein

            Jacob C. Schwartz and David R. Corey

 

21. Inhibition of Human Papillomavirus Expression Using DNAzymes

            María Luisa Benítez-Hess, Pablo Reyes-Gutiérrez, and Luis Marat Alvarez-Salas

Blurb

The field of oligonucleotide therapeutics research is ripe with the prospect of new discoveries. In Therapeutic Oligonucleotides: Methods and Protocols, a selection of established and emerging methods for the application of oligonucleotides as therapeutics are presented, all providing the tools needed to inspire great changes in the field. Divided into twenty-one chapters, this detailed volume meticulously describes vital protocols for optimizing and improving cell uptake, such as photochemical internalization, modified cell penetrating peptides, antibody conjugates, and nanoparticles. Other chapters address quantitation of RNA therapeutics in cells, assaying gene knockdown, selecting the best target site and synthesis of various modified oligonucleotides. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and easily accessible, Therapeutic Oligonucleotides: Methods and Protocols serves as a timely resource for both professionals and novices pursuing research in this exciting and pioneering field.