The Fluorescent Protein Revolution

ISBN-13: 9780367378707 / Angielski / Miękka / 2019 / 347 str.

Richard N. Day; Michael W. Davidson

Opis

This book presents the fundamental principles and latest advances in the field, including the associated development of imaging techniques that exploit fluorescent proteins. The book first traces the history of fluorescent proteins and the revolution they enabled in cellular imaging. It then discusses fluorescent proteins with novel photophysical properties. The book also covers cutting-edge imaging applications, including variations of FRAP for assessing protein dynamics, FRET microscopy of living cells, and superresolution microscopies for molecular imaging.

Spis treści

HISTORY AND PERSPECTIVES: Introduction to Fluorescent Proteins. PHOTOPHYSICAL PROPERTIES OF FLUORESCENT PROTEINS: Lessons Learned from the Structures of Fluorescent Proteins. Optimization of Fluorescent Proteins. Development of New Colors from Coral Fluorescent Proteins. Red Fluorescent Proteins: Multipurpose Markers for Live-Cell Imaging. Optical Highlighter Photophysical Properties. Far-Red and Near-Infared Fluorescent Proteins. APPLICATIONS: Genetically Encoded Fluorescent Proteins and FRAP. Optical Highlighters: Applications to Cell Biology. Optogenetic Tools Derived from Plant Photoreceptors. Fluorescent Proteins for FRET: Monitoring Protein Interactions in Living Cells. Superresolution Techniques Using Fluorescent Protein Technology. In Vivo Imaging Revolution Made by Fluorescent Proteins. Index.

O autorze

Richard N. Day, Michael W. Davidson