Techniques in Molecular Systematics and Evolution

ISBN-13: 9783764362577 / Angielski / Miękka / 2002 / 410 str.

Opis

The amount of information that can be obtained by using molecular techniques in evolution, systematics and ecology has increased eponentially since the 1990s. This manual presents some of the most important techniques for data aquisition developed.

Spis treści

I Analytical Methods.- to Part I.- 1 Phylogenetic Analysis: The Basic Method.- 2 Parsimony Analysis.- 3 Optimization Alignment: Down, Up, Error, and Improvements.- 4 Techniques for Analyzing Large Data Sets.- 5 Measures of Support.- 6 Partitioning of Multiple Data Sets in Phylogenetic Analysis.- 7 Complex Model Organism Genome Databases.- 8 Comparative Phylogenomics: A Strategy for High-throughput Large-scale Sub-genomic Sequencing Projects for Phylogenetic Analysis.- 9 Comparative Methods.- 10 Analyzing Data at the Population Level.- II Laboratory Methods.- to Part II.- 11 Obtaining, Storing and Archiving Specimens and Tissue Samples for Use in Molecular Studies.- 12 DNA Isolation Procedures.- 13 Late Pleistocene DNA Extraction and Analysis.- 14 PCR Methods and Approaches.- 15 High-Throughput DNA Sequencing for Systematics.- 16 A Practical Guide for Microsatellite Analysis.- 17 Determining the Spatial and Temporal Patterns of Developmental Gene Expression in Vertebrates and Invertebrates Using in situ Hybridization Techniques.- Guide to Solutions.- Guide to protocols.- Troubleshooting Guide.