Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols

ISBN-13: 9781627032599 / Angielski / Twarda / 2013 / 323 str.

Peter M. Rabinovich

Opis

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery. Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.

Spis treści

Part I. mRNA Synthesis, Purification, and Transfer

 

1. Cell Engineering with Synthetic Messenger RNA 

            Peter M. Rabinovich and Sherman M. Weissman

 

2. In vitro Transcription of Long RNA Containing Modified Nucleosides

Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karikó

 

3. HPLC Purification of in vitro Transcribed Long RNA

            Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karikó

 

4. Synthetic mRNAs with Superior Translation and Stability Properties

            Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads

 

5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA 

            James G. Hecker

 

6. Light-Induced mRNA Transfection  

            Sigurd Leinæs Bøe and Eivind Hovig

 

7. Engineering B Cells with mRNA 

            Jaewoo Lee, David Boczkowski, and Smita Nair

 

8. Programming Human Dendritic Cells with mRNA

            Jaewoo Lee, David Boczkowski, and Smita Nair

 

9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process

            Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa

 

10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation

            Melanie Galla, Axel Schambach, and Christopher Baum

 

11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells

            Frank Breinig, Tanja Breinig, and Manfred J. Schmitt

 

 

Part 2. Modulation and monitoring of cell metabolism      

 

12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

            Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken

 

13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors

            Noriko Shimasaki and Dario Campana

 

14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

            Peizhe Wang and Jie Na

 

15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies

            Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin

 

16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA

            Chantal Pichon and Patrick Midoux

 

17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA

            Özcan Met and Inge Marie Svane

 

18. mRNA electroporation as a Tool for Immunomonitoring

            Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman

 

19. mRNA PCR-Based Epitope Chase Method          

            Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe

 

 

Part 2. Modulation and monitoring of cell metabolism      

 

12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy

            Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken

 

13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors

            Noriko Shimasaki and Dario Campana

 

14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA

            Peizhe Wang and Jie Na

 

15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies

            Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin

 

16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA

            Chantal Pichon and Patrick Midoux

 

17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA

            Özcan Met and Inge Marie Svane

 

18. mRNA electroporation as a Tool for Immunomonitoring

            Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman

 

19. mRNA PCR-Based Epitope Chase Method          

            Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe