Structural Genomics on Membrane Proteins

ISBN-13: 9780367391102 / Angielski / Miękka / 2019 / 400 str.

Kenneth H. Lundstrom

Opis

This book presents various structural genomics approaches and explores novel technologies that will potentially accelerate breakthroughs in drug discovery. More than 40 top researchers write about their recent progress and challenges they face. They provide an excellent overview on novel research in bioinformatics, modeling on membranes, and the latest technological developments being employed in expression, purification, and crystallography to obtain high-resolution structures on membrane proteins. The book includes examples of studies on whole genomes of specific organisms and gene protein families and sections on signaling, solubilization, and recombinant expression technologies.

Spis treści

Introduction. Bioinformatics in Membrane Protein Analysis. Prokaryotic Membrane Transport Proteins: Amplified Expression and Purification. Membrane Protein Production Strategies for Structural Genomics. Refolding of Membrane Proteins for Large-scale Production. Crystallization of Membrane Proteins. Signaling through Membrane Proteins. Expression of Membrane Proteins in Yeasts. Expression of Functional Membrane Proteins in the Baculovirus-Insect Cell System: Challenges and Developments. Expression of Membrane Proteins in Mammalian Cells, Solubilization and Purification of Membrane Proteins. Fluorescent Labeling of Membrane Proteins in Living Cells. Membrane Protein NMR. Miniaturization of Structural Biology Technologies: From Expression to Biophysical Analyses. Electron and Atomic Force Microscopy of Reconstituted Membrane Proteins. Structural Genomics on Membrane Proteins. Molecular Modeling of Membrane Proteins. Towards Structural Bases for GPCR Ligand-binding: A Path for Drug Discovery.

O autorze

Kenneth H. Lundstrom