Structural Genomics and Drug Discovery: Methods and Protocols

ISBN-13: 9781493944620 / Angielski / Miękka / 2017 / 344 str.

Wayne F. Anderson

Spis treści

1. Data Management in the Modern Structural Biology and Biomedical Research Environment

Matthew D. Zimmerman, Marek Grabowski, Marcin J. Domagalski, Elizabeth M. MacLean, Maksymilian Chruszcz, and Wladek Minor

 

2. Structural Genomics of Human Proteins

Khan Tanjid Osman and Aled Edwards

 3. Target Selection for Structural Genomics of Infectious Diseases

Corin Yeats, Benoit H. Dessailly, Elizabeth M. Glass, Daved H. Fremont, Christine A. Orengo

 

4. Selecting Targets from Eukaryotic Parasites for Structural Genomics and Drug Discovery

Isabelle Q.H. Phan, Robin Stacy,  and Peter J. Myler

 

5. High Throughput Cloning for Biophysical Applications

Keehwan Kwon and Scott N. Peterson

 

6. Expression and Solubility Testing in a High Throughput Environment

Keehwan Kwon and Scott N. Peterson

 

7. Protein Production for Structural Genomics Using E. coli Expression

Magdalena Makowska-Grzyska, Youngchang Kim,Natalia Maltseva, Hui Li, Min Zhou,Grazyna Joachimiak, Gyorgy Babnigg, and Andrzej Joachimiak

 

8. Eukaryotic Expression Systems for Structural Studies

Christopher A. Nelson, William H. McCoy, and Daved H. Fremont

 

9. Automated Cell-free Protein Production Methods for Structural Studies

Emily T. Beebe, Shin-ichi Makino, John L. Markley, and Brian G. Fox

 

10. Parallel Protein Purification

Ludmilla Shuvalova

 

11. Oxidative Refolding from Inclusion Bodies

Christopher A. Nelson, Chung A. Lee, and Daved H. Fremont

 

12. High throughput Crystallization Screening

T. Skarina, X. Xu, E. Evdokimova, A. Savchenko

 

13. Screening Proteins for NMR Suitability

Adelinda A. Yee, Anthony Semesi, Maite Garcia, and Cheryl H. Arrowsmith

 

14. Salvage or Recovery of Failed Targets by in situ Proteolysis

Yufeng Tong, Aiping Dong, Xiaohui Xu, and Amy Wernimont

 

15. Salvage of Failed Protein Targets by Reductive Alkylation

 

Kemin Tan, Youngchang Kim, Catherine Hatzos-Skintges, Changsoo Chang, Marianne Cuff, Gekleng Chhor, Jerzy Osipiuk, Karolina Michalska, Boguslaw Nocek, Hao An,  Gyorgy Babnigg, Lance Bigelow, Grazyna Joachimiak, Hui Li, Jamey Mack, Magdalena M. Makowska-Grzyska, Natalia Maltseva, Rory Mulligan, Christine Tesar, Min Zhou^, and Andrzej Joachimiak

 

16. Salvage or Recovery of Failed Targets by Mutagenesis to Reduce Surface Entropy

Lukasz Goldschmidt, David Eisenberg, and Zygmunt S. Derewenda

 

17. Data Collection for Crystallographic Structure Determination

Kanagalaghatta Rajashankar and Zbigniew Dauter

 

18. Structure, Determination, Refinement, and Validation

George Minasov and Wayne F. Anderson

 

19. Virtual High-Throughput Ligand Screening

T.  Andrew Binkowski, Wei Jiang, Benoit Roux, Wayne Anderson, and Andrzej Joachimiak

 

20. Ligand Screening using Fluorescence Thermal Shift Analysis (FTS)

Chi-Hao Luan, Samuel H. Light, Sara F. Dunne, and Wayne F. Anderson

 

21. Ligand Screening using Enzymatic Assays

Kiira Ratia, Shahila Mehboob, and Hyun Lee

 

22. Ligand Screening using NMR

Benjamin E. Ramirez, Aleksandar Antanasijevic, and Michael Caffrey

 

23. Screening Ligands by X-ray Crystallography

Douglas R. Davies

 24. Case Study – Structural Genomics and Human Protein Kinases

Jonathan M. Elkins

Blurb

Structural Genomics and Drug Discovery: Methods and Protocols focuses  on high throughput structure determination methods and how they can be applied to lay the groundwork for structure aided drug discovery. The methods and protocols that are described can be applied in any laboratory interested in using detailed structural information to advance the initial stages of drug discovery.​Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Structural Genomics and Drug Discovery: Methods and Protocols seeks to aid scientists in the further study into structural genomics approach as an efficient initial step toward drug discovery and the methods described will be useful to anyone interested in moving in this direction.