Stroke Genomics: Methods and Reviews

ISBN-13: 9781617375002 / Angielski / Miękka / 2010 / 351 str.

Simon J. Read; David Virley

Opis

With sequencing of the human genome now complete, deciphering the role of gene function in human neurological pathophysiology is a promise that has yet to be realized. More than most diseases, stroke has been keenly studied from a genomic perspective. Studies are numerous and incorporate data on stroke inheritance, chromosomal loci of risk, preclinical models of stroke, and differential gene expression of brain injury, repair, and recovery. The problem is no longer a lack of information but one of interpretation and prioritization of what we do know. The aims of Stroke Genomics: Methods and Reviews are twofold. First, it aims to provide the reader with cutting-edge reviews of clinical and preclinical genomics, written by leading experts in the field. In particular, the authors of certain chapters relate gene expression changes to physiological end points, such as functional imaging paradigms. Thus, a more holistic approach to gene expression is described, one in which molecular biology goes hand in hand with stroke pathophysiology. Second, detailed methods for study of the molecular biology of stroke are also included. Following the format of the Methods in Molecular Medicine series, these chapters will enable the reader to employ each technique without recourse to other methods texts. In its entirety, this book should provide the reader with the knowledge needed to design, execute, and interpret preclinical and clinical studies of stroke genomics."

Recenzje

From the reviews:

"The protocols presented in Stroke Genomics: Methods and Reviews will greatly benefit scientists interested in the molecular biology as well as the genetic and genomic basis of strokes." - Journal of Chemical Neuroanatomy

"...very interesting and informative...an attractive and interesting overview of the different aspects of the current stroke research...can be recommended as a source of useful and up-to-date practical and theoretical information on stroke genetics and on stroke associated gene expression." - European Neurology

"This book gives the opportunity for experts in the field to explore the practical and experimental applications of genomic and molecular biology research to the treatment of stroke. ... The book also indicates detailed methods for studying the molecular biology of stroke. ... It can be recommended if one is interested in the basic scientific background that underlies central nervous system pathology ... ." (J. V. Byrne, Neuroradiology, Vol. 47, 2005)

Spis treści

Part I. Introduction Introduction to Stroke Genomics Simon J. Read and Frank C. Barone Part II. Preclinical Models and Gene Manipulation Choice, Methodology, and Characterization of Focal Ischemic Stroke Models: The Search for Clinical Relevance David Virley Mutant Animal Models of Stroke and Gene Expression: The Stroke-Prone Spontaneously Hypertensive Rat Hilary V. O. Carswell, Martin W. McBride, Delyth Graham, Anna F. Dominiczak, and I. Mhairi Macrae Gene Therapy in Neurological Disease Midori A. Yenari and Robert M. Sapolsky Stem Cell Transplantation After Middle Cerebral Artery Occlusion Paul Stroemer and Helen Hodges Endogenous Brain Protection: Models, Gene Expression, and Mechanisms Frank C. Barone Production of Transgenic and Mutant Mouse Models Alex J. Harper 3-Nitropropionic Acid Model of Metabolic Stress: Assessment by Magnetic Resonance Imaging Toby John Roberts Part III. Clinical Paradigms Practicalities of Genetic Studies in Human Stroke Ahamad Hassan and Hugh S. Markus Evaluation of the Interactions of Common Genetic Mutations in Stroke Zoltán Szolnoki Part IV. Assessing Differential Expression Technologies of Disease-Related Gene Discovery Using Preclinical Models of Stroke Xinkang Wang Quantitative Analysis of Gene Transcription in Stroke Models Using Real-Time RT-PCR David C. Harrison and Brian C. Bond Effective Analysis of Genomic Data Paul R. Nelson, Andrew B. Goulter, and Richard J. Davis Bioinformatic Approaches to Assigning Protein Function From Novel Sequence Data David Michalovich and Richard Fagan Pragmatic Target Discovery From Novel Gene to Functionally Defined Drug Target: The Interleukin-1 Story Stuart McRae Allan Indexƒ

O autorze

Leading experts explore the pragmatic application of the tools of genomic and molecular biology research to the treatment of stroke. The authors relate changes in gene expression to physiological endpoints-such as functional imaging paradigms-to produce a more holistic approach in which molecular biology goes hand-in-hand with stroke pathophysiology. The book shows how to successfully design, execute, and interpret preclinical and clinical molecular biological studies of stroke as well as rapid development of the next generation of stroke therapeutics.

Blurb

Although numerous genomic studies of stroke have examined such factors as stroke inheritance, chromosomal loci of risk, preclinical models of stroke, and differential gene expression of brain injury, repair, and recovery, much work to determine the precise role of gene function in human neurological pathophysiology remains ahead. In Stroke Genomics: Methods and Reviews, leading experts in the field explore the pragmatic application of the tools of genomic and molecular biology research to the treatment of stroke. Providing the reader with cutting-edge reviews of clinical and preclinical genomics, the authors relate changes in gene expression to physiological end points-such as functional imaging paradigms-to produce a more holistic approach in which molecular biology goes hand in hand with stroke pathophysiology. Topics of special interest include stem cell transplantation, gene therapy, clinical gene/gene interaction studies, and cytokine drug discovery. Readily reproducible techniques are provided, along with ancillary methods (imaging, animal models, human genetic study design, and transgenic animal production) that can be effectively applied to gene discovery. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Stroke Genomics: Methods and Reviews shows how to successfully design, execute, and interpret preclinical and clinical molecular biological studies of stroke, work that will greatly advance its understanding, as well as rapid development of the next generation of stroke therapeutics.