Stepwise Culture of Human Adult Stem Cells

ISBN-13: 9788770228541 / Twarda / 2024 / 144 str.

Opis

This volume on stepwise techniques to culture adult human stem cells begins with the basic methods to culture adult stem cells followed by related methods to supplement stem cell culture.

Spis treści

Adult Stem Cell 1. Isolation of Hematopoietic Stem Cells 2. Isolation Protocol for CD133+ and CD34+ Very Small Embryonic Like Cells (VSEL) from Human Umbilical Cord Blood 3. Isolation and Expansion of Mesenchymal Stem Cells from the Adult Bone Marrow Aspirate 4. Manual Isolation and Expansion of Adipose Derived Stem Cells from Adipose Tissue 5. Bone Stem Cells 6. Isolating Dental Pulp Stem Cells 7. A Murine System for Somatic Cellular Reprogramming into Induced Pluripotent Stem Cells Fetal Stem Cell 8. Isolation and Expansion Of Placenta Stem Cells (P-MSCs) 9. Isolation and Expansion of Umbilical Cord Stem Cells Cancer Stem Cell 10. Laboratory Isolation of Leukemia Cancer Stem Cell 11. Isolation and Characterization of Cancer Stem cells from Glioblastoma Multiforme and Breast Cancer Cell Lines Other Supporting Methods 12. Bone Marrow Aspiration 13. Stromal Cell Isolation 14. Isolating Mononuclear Cells by Ficoll-Hypaque Density Gradient 15. Phenotypic and Multipotent Characterization of Bone Marrow-derived Mesenchymal Stem Cells 16. Cryopreservation of Relatively Small Number of Stem Cells 17. Cryopreservation of Mobilized Peripheral Blood Stem Cells 18. Manual Cell Count

O autorze

Pranela Rameshwar is a tenured professor of medicine, Division of Hematology and Oncology at Rutgers New Jersey Medical School, Rutgers. She received her B.Sc. degree in medical microbiology from the University of Wisconsin at Madison and Ph.D. in biology from Rutgers University, New Jersey. She performed postdoctoral studies in hematopoiesis at New Jersey Medical School. Dr. Rameshwar’s research interest in stem cell biology began with her doctoral thesis and continues with research on breast cancer dormancy, neural regulation of hematopoiesis, and clinical application of adult human mesenchymal stem cells. Her research continues to be funded by federal, state, and other agencies. Dr. Rameshwar has edited books and authored >200 publications, which include original articles, reviews, editorials and book chapters. Dr. Rameshwar initiated a certificate program in stem cell biology and directs four graduate level courses in stem cell biology.