Solanum Tuberosum: Methods and Protocols

ISBN-13: 9781071616086 / Angielski / Twarda / 2021 / 408 str.

David Dobnik; Kristina Gruden; Ziva Ramsak

Spis treści

Importance of Potato as a Crop and Practical Approaches to Potato Breeding.- Cryopreservation of Potato Shoot Tips for Long Term Storage.- RNA Sequencing Analyses for Deciphering Potato Molecular Response.- Yeast-2-hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum.- Potato as a Model for Field Trials with Modified Gene Functions in Research And Translational Experiments.- DAP-seq Identification of Transcription Factor Binding Sites in Potato.- Mass Spectrometric Monitoring Of Plant Hormone Crosstalk During Biotic Stress Responses In Potato (Solanum tuberosum L.).- A Comprehensive Guide to Potato Transcriptome Assembly.- MapMan visualization of RNA-seq data using Mercator4 functional annotations.- Identification of Resistance Genes Using Diagnostic R-gene Enrichment Sequencing (dRenSeq).- Methodologies for Discovery and Quantitative Profiling of sRNAs in Potato.- Co-expression for Genotype-Phenotype Function Annotation In Potato Research.- Computer Vision and Less Complex Image Analyses to Monitor Potato Traits In Fields.- Quantifying the contribution to virulence of Phytophthora infestans effectors in potato.- Identification of Solanum immune receptors by Bulked Segregant RNA-Seq and high-Throughput Recombinant Screening.- Gene editing in potato using CRISPR-Cas9 technology.- Gene downregulation in potato roots using Agrobacterium rhizogenes-mediated Transformation.- Molecular Detection of Ralstonia solanacearum to Facilitate Breeding for Resistance to Bacterial Wilt in Potato.- Towards the design of potato tolerant to abiotic stress.- Rapid loop-mediated isothermal amplification for detection of the Ralstonia solanacearum species complex bacteria in symptomatic potato tubers and plants.

 

Blurb

This volume offers a comprehensive collection of experimental approaches to study Solanum tuberosum (potato). Chapters are divided into five sections covering a general overview of potato, the research fields of  molecular biology, omics approaches, and bioinformatics, and finally, the application of the generated knowledge for crop improvement. Written in the format of the highly successful Methods in Molecular Biology series, each wet-lab chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and practical, Solanum tuberosum: Methods and Protocols aims to provide a collection of methods for the potato research community that will enable scientists gain insight into the world of the potato.

Chapter 2 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.