Site-Specific Protein Labeling: Methods and Protocols

ISBN-13: 9781493944170 / Angielski / Miękka / 2016 / 267 str.

Arnaud Gautier; Marlon J. Hinner

Opis

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step.

Spis treści

1. How FlAsH got its Sparkle:Historical Recollections of the Biarsenical-tetracysteine Tag

B. Albert Griffin, Stephen R. Adams, and Roger Y. Tsien

 

2. Site-Specific Protein Labeling in the Pharmaceutical Industry - Experiences from Novartis Drug Discovery

Lukas Leder

 

3. Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins

Nicole J. Yang and Marlon J. Hinner

 

4. Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes

Ivan R. Corrêa Jr.

 

5. 2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues

Lina Cui and Jianghong Rao

 

6. Fluorescent Labeling for Patch Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels

Thomas K. Berger and Ehud Y. Isacoff

 

7. Fluorescent Labeling Of SNAP-Tagged Proteins In Cells

Gražvydas Lukinavičius, Luc Reymond, and Kai Johnsson

 

8. HaloTag Technology For Specific And Covalent Labeling Of Fusion Proteins

Hélène A. Benink and Marjeta Urh

 9. Ligation Of Synthetic Peptides To Proteins Using Semisynthetic Protein Trans-Splicing

Julian Matern, Anne-Lena Bachmann, Ilka V. Thiel, Gerrit Volkmann, Alexandra Wasmuth, Jens Binschik and Henning D. Mootz

 10. Chemical-tag Labeling Of Proteins Using Fully Recombinant Split Inteins

Anne-Lena Bachmann, Julian Matern, Vivien Schütz, and Henning D. Mootz

 

11. Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase Catalyzed Site-Specific Protein Labeling

Bo Zhao, Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Han Zhou, Mengnan Zhang, and Jun Yin

 

12. Site-Specific Biotinylation Of Purified Proteins Using BirA

Michael Fairhead and Mark Howarth

 

13. Site-specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist

Maximilian Wei-Lin Popp

 

14. BONCAT – Metabolic Labeling, Click Chemistry, and Affinity Purification Of Newly Synthesized Proteomes

Peter Landgraf, Elmer R. Antileo, Erin M. Schuman, and Daniela C. Dieterich

 

15. Genetic Encoding Of Unnatural Amino Acids For Labeling Proteins

Kathrin Lang, Lloyd Davis,  and Jason W. Chin

 

16. Labeling Proteins By Affinity-Guided DMAP Chemistry

Tomonori Tamura and Itaru Hamachi

 

17. Ligand-Directed Tosyl Chemistry For Selective Native Protein Labeling In Vitro, In Cells, and In Vivo

Shinya Tsukiji and Itaru Hamachi

Blurb

This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information regarding the design and generation of the organic molecules used for the labeling step. Chapters present a comprehensive overview on the most relevant and established labeling methodologies, and help researchers to choose the most appropriate labeling method for their biological question. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols is designed to help scientists from all fields to get a better understanding of labeling techniques.