Signal Transduction Protocols

ISBN-13: 9781493961085 / Angielski / Miękka / 2016 / 430 str.

Louis M. Luttrell; Stephen S. G. Ferguson

Opis

It is increasingly clear that signal transduction is a highly organized and integrated process. Divided into two convenient sections, this book focuses on experimental approaches to better understand the complexity of signal transduction.

Recenzje

From the reviews of the third edition:

"This well-illustrated book on signal transduction protocols is a valuable resource for scientists interested in learning the intricate details of complex signaling events that take place within the cell ... . Although written for the scientists in the field of signal transduction research, it also will be useful for graduate students and other investigators in pharmacology interested in this area. ... Given the rapid advances in the field of signal transduction, this third edition is essential." (Omer Iqbal, Doody's Book Reviews, March, 2012)

Spis treści

Part I.  Overviews

 

1. Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling

            Louis M. Luttrell and Terry P. Kenakin

 

2. Imaging-based Approaches to Understanding G Protein-Coupled Receptor Signaling Complexes

            Darlaine Pétrin and Terence E. Hébert

 

3. Improving Drug Discovery with Contextual Assays and Cellular Systems Analysis

            John K. Westwick and Jane E. Lamerdin

 

4. RGS-insensitive Ga Subunits: Probes of G Subtype-selective Signaling and Physiological Functions of RGS Proteins

            Kuljeet Kaur, Jason M. Kehrl, Raelene A. Charbeneau, and Richard R. Neubig

 

5. Bioinformatic Approaches to Metabolic Pathways Analysis

            Stuart Maudsley, Wayne Chadwick, Liyun Wang, Yu Zhou, Bronwen Martin, and Sung Park

 

Part II. Receptor – Ligand Interactions

 

6. Studying Ligand Efficacy at G Protein-Coupled Receptors Using FRET

            Jean-Pierre Vilardaga

 

7. Using BRET to Detect Ligand-Specific Conformational Changes in Preformed Signaling Complexes

            Nicolas Audet and Graciela Piñeyro

 

Part III. Receptor – Receptor Interactions

 

8. Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High Density Lipoprotein Particles (rHDL)

            Gisselle A. Vélez-Ruiz and Roger K. Sunahara

 

9. Using Quantitative BRET to Assess G Protein-Coupled Receptor Homo- and Hetero-Dimerization

            Lamia Achour, Maud Kamal, Ralf Jockers, and Stefano Marullo

 

10. Cell-Surface Protein-Protein Interaction Analysis with Time-Resolved FRET and Snap-tag Technologies: Application to G Protein-Coupled Receptor Oligomerization

            Laëtitia Comps-Agrar, Damien Maurel, Philippe Rondard, Jean-Philippe Pin, Trinquet Eric, and Laurent Prézeau

 

11. Analysis of GPCR/Ion Channel Interactions

            Christophe Altier and Gerald W. Zamponi

 

Part IV. Receptor – Effector Coupling

 

12. Multicolor BiFC Analysis of G Protein  Complex Formation and Localization

            Thomas R. Hynes, Evan A. Yost, Stacy M. Yost, and Catherine H. Berlot

 

13. Real-Time BRET Assays to Measure G Protein/Effector Interactions

            Darlaine Pétrin, Mélanie Robitaille, and Terence E. Hébert

 

14. Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP

            Brock F. Binkowski, Frank Fan, and Keith V. Wood

 

15. Simultaneous Real Time Imaging of Signal Oscillations Using Multiple Fluorescence-Based Reporters

            Lianne B. Dale and Stephen S. G. Ferguson

 

Part V. Spatial Control of Signal Transduction

 

16. Using FRET-based Reporters to Visualize Subcellular Dynamics of Protein Kinase A Activity

            Charlene Depry and Jin Zhang

 

17. Genetically Encoded Fluorescent Reporters to Visualize Protein Kinase C Activation in Live Cells

            Lisa L. Gallegos and Alexandra C. Newton

 

18. Visualizing Receptor Endocytosis and Trafficking

            Ali Salahpour and Larry S. Barak

 

19. Investigating G Protein-Coupled Receptor Endocytosis and Trafficking by TIR-FM

            Guillermo A. Yudowski and Mark von Zastrow

 

20. Visualizing G Protein-Coupled Receptor Signalsomes Using Confocal Immunofluorescence Microscopy

            Sudha K. Shenoy

 

Part VI. Protein – Protein Interactions

 

21. Detection and Characterization of Receptor Interactions with PDZ Domains

            Stefanie L. Ritter and Randy A. Hall

 

22. Tandem Affinity Purification and Identification of Heterotrimeric G-Protein Associated Proteins

            Syed M. Ahmed, Avais M. Daulat, and Stéphane Angers

 

23. Study of G Protein-Coupled Receptor/ßarrestin Interactions within Endosomes Using FRAP

            Benjamin Aguila, May Simaan, and Stéphane A. Laporte

 

24. Disrupting Protein Complexes Using Tat-tagged Peptide Mimics

            Shupeng Li, Sheng Chen, Yu Tian Wang, and Fang Liu

 

25. Protein-fragment Complementation Assays for Large-Scale Analysis, Functional Dissection and Dynamic Studies of Protein-Protein Interactions in Living Cells

            Stephen W. Michnick, Po Hien Ear, Christian Landry, Mohan K. Malleshaiah, and Vincent Messier

Blurb

It is increasingly clear that signal transduction is a highly organized and integrated process.  Divided into two convenient sections, Signal Transduction Protocols, Third Edition focuses on experimental approaches to better understand the complexity of signal transduction.  Introductory chapters provide perspective on several of the challenges involved in signal transduction research and offer guidance on selecting the best approaches to various types of questions.  The individual chapters provide detailed experimental protocols covering various topics, from the effects of ligand binding on receptor conformation and effector coupling, to moving inside the cell in order to capture the spatial and temporal characteristics of signaling events. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Signal Transduction Protocols serves as an ideal guide to scientists of all backgrounds and is a valuable resource for future progress in the field of signal transduction research.