Sensors and Probes for Bioimaging

ISBN-13: 9783527349609 / Angielski / Twarda / 2023 / 300 str.

Y-T Chang

Spis treści

1 INTRODUCTION OF BIOIMAGING1.1. Color1.2 Colorful material1.3 Light source of bioimaging1.4 Subcellular imaging1.5 Cell selective imaging1.6 Tissue and organ imaging1.7 Whole body imaging1.8 Probes in bioimaging2. CHEMICAL SENSORS AND PROBES FOR BIOIMAGING2.1 History of dyes in biological stains2.2 Blood cell staining2.3 Bacteria staining using Gram method2.4 Fluorescent sensors and probes2.5 Representative fluorescent compounds for bioimaging3. ORGANELLE SELECTIVE PROBES3.1 Cell plasma membrane3.2 Endosome and lysosome3.3 Nucleus and DNA3.4 Nucleolus and RNA3.5 ER and Golgi body3.6 Mitochondria3.7 Lipid droplet3.8 Peroxisome3.9 Cytosol3.10 Extracellular vesicle3.11 Nonmembrane-bound condensate3.12 Organelle probes in live cells and fixed cells3.13 Modeling for organelle selective probes4. LIVE CELL SELECTIVE PROBES4.1. Protein Oriented Live Cell Distinction (POLD)4.2. Carbohydrate Oriented Live Cell Distinction (COLD)4.3. Lipid Oriented Live Cell Distinction (LOLD)4.4. Gating Oriented Live Cell Distinction (GOLD)4.5. Metabolism Oriented Live Cell Distinction (MOLD)5. EX-VIVO TISSUE SELECTIVE PROBES5.1 Immunohistochemistry5.2 Tissue imaging with nucleic acid probes5.3 Tissue imaging with small molecule probes5.3.1 Pancreatic islet imaging5.3.2 Neuronal tissue imaging5.4 Organoid as model of tissue and organ6. IN-VIVO WHOLE-BODY IMAGING PROBES6.1 ElaNIR for elastin imaging in mouse6.2 Probes for exposed neuron in zebrafish embryo6.3 NeuO for whole-body neuron imaging in zebrafish6.4 LipidGreen for fatty tissue imaging in zebrafish6.5 Blood vessel imaging in zebrafish6.6 Probes for bone imaging6.7 Probes for pancreatic islet imaging6.8 Probes for eye imaging6.9 Macrophage imaging in ischemia and inflammation7. IMAGING FOR BIOLOGICAL ENVIRONMENT AND FUNCTION7.1 pH7.2 Metal ions7.3 Metabolites7.4 Viscosity7.5 Temperature7.6 ROS and RNS8. IMAGING FOR DISEASE8.1 Cancer imaging8.2 Neurodegenerative disease imaging8.3 Inflammation imaging8.4 Diabetes imaging8.5 Liver disease imaging8.6 Aging8.7 Theranostics9. NON-OPTICAL IMAGING PROBES9.1 Ultrasound imaging probes9.2 X-ray contrast agents9.3 MRI contrast agents9.4 SPECT probes9.5 PET probes9.6 Multimodality10. FLUORESCENCE IMAGING TECHNIQUES AND ANALYSIS METHODS10.1 Multi-color imaging10.2 Ratiometric measurement10.3 Fluorescence lifetime imaging microscopy10.4 Confocal fluorescence microscopy10.5 Two photon excitation fluorescence imaging and harmonic generation10.6 Selective plane illumination microscopy10.7 Total internal reflection fluorescence microscopy10.8 Super resolution imaging10.9 Single molecule imaging10.10 Photoactivation of caged molecule10.11 Fluorescence recovery after photobleaching10.12 Flow cytometry technique11. PERSPECTIVES FOR FUTURE PROBE DEVELOPMENT11.1 Design of selective probes11.2 Discovery of selective probes by screening11.3 Future probe development

O autorze

Young-Tae Chang studied chemistry in Pohang University of Science and Technology (POSTECH, Korea) and received his B.S. in 1991. After one and half years of army service in Korea, he started his graduate study at POSTECH and received a Ph.D. in 1997 under the supervision of Prof. Sung-Kee Chung, working on the divergent synthesis of all possible regioisomers of myo-inositol phosphates. He did his postdoctoral work with Prof. Peter Schultz at UC Berkeley and The Scripps Research Institute. In 2000, he was appointed assistant professor at New York University and promoted to associated professor in 2005. In September, 2007, he moved to National University of Singapore and Singapore Bioimaging Consortium. He was a full professor of Chemistry and leader of Medicinal Chemistry Program of NUS, and Lab Head of Bioimaging Probe Development at SBIC, Biopolis. In 2017, he came back to POSTECH, as a professor in the Department of Chemistry, and joined Center for Self-assembly and Complexity (CSC), Institute for Basic Science (IBS) as an Associate Director. He published more than 300 scientific papers / 2 books and filed more than 50 patents so far.Nam-Young KANG is a Research Professor at the Department of Convergence IT Engineering, Pohang University of Science and Technology (POSTECH). Her research of interest is focused on Cell image-based High contents/through-put screening (HCS/HTS) to develop HCS based new drug using fluorescent imaging probes.