"This monograph summarises and presents in an organised single text scattered relevant background information concerning sample preparation for a variety of molecular biology analyses, together with a number of protocols for successful treatment of difficult matrices. This book can be considered a useful practical guide for all those involved in sample preparation within the molecular biology practice, but it is also suitable for newcomers trying to obtain a general, but complete overview of the subject." (Lourde Ramos, Chromatographia, Vol. 79, 2016)
Contents
Foreword
Ljubisav Rakic
1. Physical and Chemical Properties of Nucleic Acids
Daphne Real
2. Sampling for Biostatistics
Angela Conley and Jason Pfefferkorn
3. Sampling of Microbiological Samples
Christina Stam, Alberto Behar, and Moogega Cooper
4. Techniques for Nucleic Acid Purifications from Plant, Animal, and Microbial Samples
Jordan Ruggieri, Ryan Kemp, Stanislav Forman, and Marc E. Van Eden
5. Magnetic beads based nucleic acids purification for molecular biology applications
Nives Kovacevic
6. Systems for Mechanical Sample Lysis and Homogenization for Molecular Biology Applications
James A. Atwood III
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7. Bead beating offers high performance homogenization for molecular biology downstream processing of tough and difficult samples
Vanessa M. Yu
8. High Performance Bead Beating Based Lysing, Homogenization and Grinding for DNA, RNA and
proteins extraction with FastPrep® Systems
Miodrag Micic, Jeffrey D. Whyte, and Véronique Karsten
9. Portable Systems for Sample Lysis and Homogenization
Horacio Kido
10. DNA and RNA extractions from mammalian samples
Shuko Harada
11. Sample preparation for multiplex PCR assays for food and agriculture applications
Rachel Binet and Aparna Tatavarthy
12. Nucleic acid sample preparation from stem cells
Mirjana Pavlovic
13. Nucleic Acids Sample Preparation from Teeth/Dental Remains
Denice Higgins and Jeremy J. Austin
14. Nucleic Acids Sample Preparation for Food Traceability
Leonor Pereira, Sónia Gomes, and Paula Martins-Lopes
15. Nucleic Acids Sample Preparation for Quantitative and Qualitative GMO analysis
Maher Chaouachi
16. Nucleic Acids Samples Preparation from Dairy Products and Milk
Julie Ann Kase and Tina Lusk
17. Extraction of DNA from
Plant Tissue: Review and Protocols
Farshad Tamari and Craig S. Hinkley
18. DNA Extraction from Seeds
Célio Dias Santos Júnior, Natália Melquie Monteiro Teles, Denis Prudencio Luiz, and Thais Ferreira Isabel
19. RNA extractions from difficult to prepare and high starch content seeds
20. Nucleic Acid and Protein Sample Preparation from Yeasts
Zhaohua Tang and Gretchen Edwalds-Gilbert
21. Nucleic acids purification from soil and environmental sources
Moogega Cooper and Christina Stam
22. Extraction and Purification of Viral Nucleic Acids from Environmental Samples
Brandon C. Iker, Masaaki Kitajima, and Charles P. Gerba<
23. Preparation of nucleic acids from marine samples: applications to microbial ecology research
Karine Lemarchand, Thomas Pollet, Vincent Lessard, and M. Amine Badri
24. Nucleic Acid Sample Preparation from Feces and Manure
Tim Reuter and Rahat Zaheer
25.
Bead-beating sample preparation for nucleic acids isolation from fecal samples
Jeffrey D. Whyte
26. Methods for extraction and purification of lignin and cellulose from plant tissues
Ksenija Radotić and Miodrag Micic
27. Separation and purification of glycans out of glycoproteins
Olga Gornik, Toma Keser and Gordan Lauc
28. Prion extraction methods: comparison of bead beating, ultrasonic disruption and repeated freeze-thaw methodologies for the recovery of functional Renilla-prion fusion protein from bacteria
Robert M. Hnasko, Alice V. Lin, Larry H. Stanker, Kumar Bala, and Jeffery A. McGarvey
Miodrag “Mickey” Mićić , Ph.D., Sc.D., M.T.M., C.S.W.P. is the Department Chair and Professor at the Department of Engineering Design Technology and Department of New Products Development at Cerritos College. He is also a Project Scientist at the BioMEMS Lab, Department of Mechanical and Aerospace Engineering, University of California, Irvine. He is a Visiting Professor at the Faculty of Applied Ecology—Futura at Singidunum University, Belgrade, Serbia. Dr. Mićić is a consultant to several life sciences, medical devices, and biotech companies. Prior to his current position at Cerritos College, Dr. Mićić was the Vice President of Research and Development at MP Biomedicals LLC (Formerly ICN Biomedicals Inc), Irvine, CA, USA. Earlier in his career, he was a Senior Application Scientist at Veeco Metrology, Santa Barbara, CA, USA. Dr. Mićić did his postdoctoral work as a Postdoctoral Research Fellow at Pacifi c Northwest National Laboratories, Richland, WA, USA. He is the author of 67 publications in leading scientific journals, with over 1,400 citations. Dr. Mićić holds a Ph.D. and M.S. degrees in Chemistry from the University of Miami, Coral Gables, FL, USA. He holds an M.T.M. degree from Washington State University, WA, USA. Further, Dr. Mićić holds Sc.D. and M.Sc. degrees in Physical Chemistry; a B.Sc./B.Ed. in Physics and Fundamentals of Engineering from the University of Belgrade, Belgrade, Serbia; and a B.Eng. degree in Mechanical/Naval Architecture Engineering from the Polytechnic Academy of New Belgrade, Belgrade, Serbia. He is also an FAA licensed instrument-rated commercial pilot and a PADI master scuba diver.
The Sample Preparation Techniques for Environmental, Plant, and Animal Samples handbook is a collection of best practices, recipes and theoretical information aimed at anyone who works with any type of molecular biology, proteomics, or metabolomics research involving diffi cult and tough-to-process samples, and thus is exposed to the seemingly unbreakable bottleneck of sample preparation. Th is book is most useful to researchers preparing nucleic acids and proteins from environmental (e.g., soil, marine, and wastewater, feces) and tough microbiological (e.g., spores, yeasts, gram positive bacteria) samples, as well as solid tissue samples from plants and animals. This book is the first comprehensive piece of literature dealing with applications of bead beating technology and other types of mechanical homogenization sample preparation.