Salmonella: Methods and Protocols

ISBN-13: 9781617376740 / Angielski / Miękka / 2010 / 364 str.

Heide Schatten; Abe Eisenstark

Opis

Salmonella: Methods and Protocols presents detailed methods on a variety of aspects of Salmonella research, focusing on those which provide landmarks for future discovery. Highlighted topics include molecular genotyping, rapid and accurate new methods for sensitive determination of antibiotic resistance profiles, the characterization of Salmonella-specific cell antigens, and Salmonella-host cell interactions. Detailed methods for studying Salmonella motility are also presented. Each chapter provides a short overview of the topic, which is followed by detailed explanations of techniques such as genetic manipulation and molecular imaging that offer the possibilities of using Salmonella as a therapeutic vector to interfere with disease. This is the first comprehensive volume of methods and protocols for studying Salmonella and will be indispensable to researchers engaged in the study of Salmonella, and enterobacteria in general. Because no recent comprehensive literature of this format is available on Salmonella, Salmonella: Methods and Protocols will be of value to a wide variety of researchers engaged in the study of Salmonella, and enterobacteria in general.

Recenzje

From the reviews:

"This book presents detailed methods and protocols for the application of various analytical techniques to Salmonella research. It is aimed at geneticists, microbiologists, public health scientists, researchers and students. The book contains 17 chapters ... ." (International Food Information Service, December, 2007)

"This is an interesting and very informative book that covers a broad range of methods in Salmonella research. Each chapter contains a useful introduction outlining in which field of research the methods are most likely to be useful. ... Clear diagrams and photographic illustrations enrich the written text material. This book is not simply a list of laboratory methods, but it is also a valuable guide that will allow bench scientists to choose the most appropriate methodological approach to solve specific research questions." (Pietro Mastroeni, Microbiology Today, May, 2008)

"The book provides quite a wide range of protocols from different fields of Salmonella research which in most cases can also be generalized for other members of the family Enterobacteriaceae. Each chapter provides a short overview of the topic which is followed by detailed protocol description. ... for those interested in entering an interesting field of Salmonella research I can recommend this book as a valuable source of a wide range of protocols enabling anybody to start from the very beginning." (I. Rychlík, Folia Microbiologia, Vol. 53 (3), 2008)

Spis treści

Table of Contents 1. Quantitative, Multiplexed Detection of Salmonella and Other Pathogens by Luminex Sherry A. Dunbar and James W. Jacobsen 2. Detection of Salmonella by Bacteriophage Felix 01 Jonathan C. Kuhn 3. Physical Mapping of Salmonella Genomes Shu-Lin Liu 4. Cloth-based Hybridization Array System for the Identification of Antibiotic Resistance Genes in Salmonella Burton W. Blais and Martine Gauthier 5. Genome-based Identification of Molecular Analyses of Pathogenicity Islands and Genomic Islands in Salmonella enterica Michael Hensel 6. Determination of the Gene Content of Salmonella Genomes by Microarray Analysis Steffen Porwollik and Michael McClelland 7. In-Vivo Excision, Cloning, and Broad-Host-Range Transfer of Large Bacterial DNA Segments Using VEX-Capture James A. Wilson and Cheryl A. Nickerson 8. Amplified Fragment Length Polymorphism Analysis of Salmonella enterica Ruiting Lan and Peter R. Reeves 9. Salmonella Phages and Prophages - Genomics and Practical Aspects Andrew M. Kropinski, Alexander Sulakvelidze, Paulina Konczy, and Cornelius Poppe 10. Salmonella Typhimurium Phage Typing for Pathogens Wolfgang Rabsch 11. Salmonella Phages Examined in the Electron Microscope Hans-W. Ackermann 12. Applications of Cell Imaging in Salmonella Research Charlotte A. Perrett and Mark A. Jepson 13. Analysis of Kinesin Accumulation on Salmonella-containing Vacuoles Audrey Dumont, Nina Schroeder, Jean-Pierre Gorvel, and Stéphane Méresse 14. Magnesium, Manganese and Divalent Cation Transport Assays in Intact Cells Michael E. Maguire 15. Methods in Cell-to-Cell Signaling in Salmonella Brian M. M. Ahmer, Jenee N. Smith, Jessica L. Dyszel, and AmberLindsay 16. Development of Salmonella Strains as Cancer Therapy Agents and Testing in Tumor Cell Lines AbrahamEisenstark, Robert A. Kazmierczak, Alison Fea, Rula Khreis, Dustin Newman, and Heide Schatten 17. Further Resources for Molecular Protocols in Salmonella Research Abraham Eisenstark and Kelly K. Edwards