Rotaviruses: Methods and Protocols

ISBN-13: 9781617371608 / Angielski / Miękka / 2010 / 262 str.

James Gray; Ulrich Desselberger

Opis

James Gray and Ulrich Desselberger have assembled a comprehensive collection of established and cutting-edge methods for studying and illuminating the structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of infant morbidity and mortality. Presented by experts in the fields of animal and human rotavirus infections and rotavirus vaccine research, these readily reproducible methods detail molecular and other modern techniques, and include relevant background information and various notes to ensure reproducible and robust results. Authoritative and up-to-date, Rotaviruses: Methods and Protocols offers researchers today's benchmark compendium of experimental methods for the investigation of this medically significant virus.

Recenzje

"This book will be very useful to both new investigators in the field and persons who have a general interest in rotaviruses and the methods to be used in their study. Many chapter contributors also provide very useful summaries of the methods used in a specific aspect of rotavirus research. These will be helpful to even the most experienced investigator in the field. "-Doody's Health Sciences Book Review Journal

"This book has no recent equivalent....The book will not only be profitable for the RV specialist; people interested in other viruses or cellular physiology will surely benefit from the lessons learned from studies of RV electron cryomicroscopy, RV replication or animal models of RV infections." - Trends in Microbiology

"...methods, which have proven invaluable to studies, are given in detail in this excellent new book. The contributors have written detailed protocols for, predominantly, molecular and serological methods with emphases on those that are somewhat unique to rotavirus research....However, there is much more to this book than the highly detailed protocols....The last of the central chapters is by Mary Ramsay and David Brown and describes the epidemiology of rotavirus infections and concentrates on surveillance and disease burden. Anyone still unconvinced of the need for a rotavirus vaccine should read this chapter."-J. Med. Microbiol

"This high quality compendium comprises 12 concise chapters, each presented in the same easy to follow layout....this book at $89.50 is reasonable value and an essential reference for any laboratory undertaking rotavirus research."- Microbiology Today

Spis treści

Rotaviruses: Basic Facts, Ulrich Desselberger. Electron Cryomicroscopy and Computer Image Processing Techniques: Use in Structure-Function Studies of Rotavirus, B. V. Venkataram Prasad and Mary K. Estes. Virus Replication, John T. Patton, Vladimir Chizhikov, Zenobia Taraporewala, and Dayue Chen. Rotavirus Entry into Tissue Culture Cells, Joanna M. Gilbert and Harry B. Greenberg. Mixed Infections with Rotaviruses: Protocols for Reassortment, Complementation, and Other Assays, Robert F. Ramig. Pathogenesis and Animal Models, Linda J. Saif and Lucy A. Ward. Immunologic Methods and Correlates of Protection, Kristine K. Macartney and Paul A. Offit. In Vivo Study of Immunity to Rotaviruses: Selected Methods in Mice, Manuel A. Franco and Harry B. Greenberg. Evaluation of Rotavirus Vaccines in Small Animal Models, Max Ciarlet and Margaret E. Conner. Methods of Rotavirus Detection, Sero- and Genotyping, Sequencing, and Phylogenetic Analysis, Miren Iturriza Gómara, Jon Green, and Jim Gray. Epidemiology of Group A Rotaviruses: Surveillance and Burden of Disease Studies, Mary Ramsay and David Brown. Future Rotavirus Research, Ulrich Desselberger and Mary K. Estes. Index.

Blurb

In Rotaviruses: Methods and Protocols, James Gray and Ulrich Desselberger have assembled a comprehensive collection of established and cutting-edge methods for studying and illuminating the structure, molecular biology, pathogenesis, epidemiology, and prevention in animal models of infection with rotaviruses, an important cause of infant morbidity and mortality. Presented by experts in the fields of animal and human rotavirus infections and rotavirus vaccine research, these readily reproducible methods detail molecular and other modern techniques, and include relevant background information and various notes to ensure reproducible and robust results. Authoritative and up-to-date, Rotaviruses: Methods and Protocols offers researchers today's benchmark compendium of experimental methods for the investigation of this medically significant virus.