Rnai: Design and Application

ISBN-13: 9781617378195 / Angielski / Miękka / 2011 / 270 str.

Sailen Barik

Opis

RNA interference (RNAi), in which RNA silences RNA, is the most recent discovery to revolutionize the study of biology. In RNAi: Design and Applications, leaders in the field contribute state-of-the-art, easy to follow methods and bench protocols designed for practical, everyday use of RNAi in biological research. Divided into two parts, this comprehensive volume covers fundamentals including designs of RNAi, biochemical assay protocols for the major components of RNAi, and study of potential off-target effects, followed by an extensive section covering various applications of RNAi in diverse model organisms and systems, from antiviral and anticancer applications to altering flower color in plants. Cutting edge and clearly written, RNAi: Design and Applications enables a researcher with standard molecular biological training to perform major RNAi-related experiments and contribute to this revolutionary, growing field.

Spis treści

Contents Preface ……………………………………………………………………………….. Contributors ………………………………………………………………………….. Part I. Designing Optimal RNAi Tools 1 Principles of Dicer Substrate (D-siRNA) Design and Function Mohammed Amarzguioui and John J. Rossi ..………………….......... 2 Expression, Purification, and Analysis of Recombinant Drosophila Dicer-1 and Dicer-2 Enzymes Xuecheng Ye and Qinghua Liu …………………………………………. 3 In vitro RNA Cleavage Assay for Argonaute-Family Proteins Keita Miyoshi, Hiroshi Uejima, Tomoko Nagami, Haruhiko Siomi, and Mikiko C. Siomi ……………………………………………………….. 4 Identifying siRNA-Induced Off-Targets by Microarray Analysis Emily Anderson, Queta Boese, Anastasia Khvorova, and Jon Karpilow ……………………………………………………………………... Part II. Application of RNAi in diverse organisms 5 Hydrodynamic Delivery of siRNA in a Mouse Model of Sepsis Doreen E. Wesche-Soldato, Joanne Lomas-Neira, Mario Perl, Chun-Shiang Chung, and Alfred Ayala ..………………………………………. 6 Nasal Delivery of siRNA Vira Bitko and Sailen Barik ..……………………………………………… 7 RNA Interference as a Genetic Tool in Trypanosomes Vivian Bellofatto and Jennifer B. Palenchar …..……………………… 8 Lentivirus-Mediated RNA Interference in Mammalian Neurons Scott Q. Harpera and Pedro Gonzalez-Alegre …...…………………… 9 Silencing Genes by RNA Interference in the Protozoan Parasite Entamoeba histolytica Carlos F. Solis and Nancy Guillén……………………….……………… 10 Use of RNAi in C. elegans Tsuyoshi Ohkumo, Chikahide Masutani, Toshihiko Eki, and Fumio Hanaoka ……….…………………………………………………………….. 11 Application of siRNA against SARS in the Rhesus Macaque Model Qingquan Tang, Baojian Li, Martin Woodle, Patrick Y. Lu ….….…… 12 siRNA and shRNA as Anticancer Agents in a Cervical Cancer Model Wenyi Gu, Lisa Putral, and Nigel McMillan …..…………………..…… 13 Identification and Expression Analysis of Small RNAs During Development Toshiaki Watanabe, Hiroshi Imai and Naojiro Minami ……………… 14 Screening and Identification of Virus-Encoded RNA Silencing Suppressors Sumona Karjee, Mohammad Nurul