Rna-RNA Interactions: Methods and Protocols

ISBN-13: 9781493918959 / Angielski / Twarda / 2014 / 219 str.

Frank J. Schmidt

Opis

In this volume" "expert researchers in the field detail many of the methods which are now commonly used to study RNA. These methods are presented as a guidebook to scientists who are experienced with RNA research and want to brush up on a new technique. Written in the highly successful "Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Thorough and intuitive, "RNA-RNA Interactions: Methods and Protocols "guides scientists investigating biological systems and studying RNA.

Spis treści

1. Identifying RNA Recombination Events and Non-covalent RNA-RNA Interactions with the Molecular Colony Technique

Helena V. Chetverina and Alexander B. Chetverin

 

2. RNA-directed Recombination of RNA in Vitro

Niles Lehman, Nilesh Vaidya, and Jessica A. M. Yeates

 

3. RNA-RNA Selex

B. Cho

 

4. Identification of Antisense RNA S0tem-loops that Inhibit RNA-protein Interactions using a Bacterial Reporter System

Kazuo Harada

 

5. Trans-activation of Large Ribozymes

Matthew B. Martin, Thomas L. Leeper, and Frank J. Schmidt

 

6. Native Purification and Labeling of RNA for Single Molecule Fluorescence Studies

Arlie J. Rinaldi, Krishna C. Suddala,and Nils G. Walter

 

7. Single Molecule Studies of RNA-RNA Interactions

Dongmei Yu, Peiwu Qin, and Peter V. Cornish

 

8. Modification Interference Analysis of the Ribosome

Simpson Joseph

 

9. Assessing Intermolecular RNA:RNA Interactions Within a Ribonucleoprotein Complex Using Heavy Metal Cleavage Mapping

Keith T. Gagnon and  E. Stuart Maxwell

 

10. Monitoring RNA-RNA Interactions With Electrophoretic Mobility Shift Assays: Analysis Of Trna Binding To The T Box Riboswitch Antiterminator RNA

R. Anupam, S. Zhou,  and  J.V. Hines

 

11. Electrophoretic Mobility Shift Assay of RNA-RNA Complexes

Geunu Bak, Kook Han, Kwang-sun Kim, and Younghoon Lee

 

12. Structural Studies of a Double-stranded RNA from Trypanosome RNA Editing by

Small Angle X-ray Scattering

Angela Criswell and Blaine H.M. Mooers

 

13. Fusion RNAs in Crystallographic Studies of Double-stranded RNA from

Trypanosome RNA Editing

Blaine H.M. Mooers