Ribosome Display and Related Technologies: Methods and Protocols

ISBN-13: 9781493958726 / Angielski / Miękka / 2016 / 424 str.

Julie A. Douthwaite; Ronald H. Jackson

Opis

Display technologies are a powerful biological tool, yet the complexity of ribosome display methods has inhibited their use. This volume provides detailed and easily followed protocols with the aim of facilitating a greater uptake of these methods.

Spis treści

Part I: Review

 

1. Ribosome Display: A Perspective

            Andreas Plückthun

 

Part II: Translation Extract Preparation

 

2. Preparation and Testing of E.coli S30 In Vitro Transcription Translation Extracts

            James F. Zawada

 

Part III: Basic Ribosome Display and Related Selection Methods

 

3. Eukaryotic Ribosome Display Selection Using Rabbit Reticulocyte Lysate

            Julie A. Douthwaite

 

4. Stabilized Ribosome Display for In Vitro Selection

            Shuta Hara, Mingzhe Liu, Wei Wang, Muye Xu, Zha Li, and Yoshihiro Ito

 

5. Eukaryotic Ribosome Display with In Situ DNA Recovery

            Mingyue He, Bryan M. Edwards, Damjana Kastelic, and Michael J. Taussig

 

6. mRNA Display Using Covalent Coupling of mRNA to Translated Proteins

            Rong Wang, Steve W. Cotten, and Rihe Liu

 

7. SNAP Display: In Vitro Protein Evolution in Microdroplets

            Miriam Kaltenbach and Florian Hollfelder

 

8. cDNA Display: Rapid Stabilization of mRNA Display

            Shingo Ueno and Naoto Nemoto

 

Part IV: Applications of Ribosome Display Methods Using Natural Amino Acids

 

9. Optimization of Antibody Affinity by Ribosome Display Using Error-Prone or Site-Directed Mutagenesis

            Leeanne Lewis and Chris Lloyd

 

10. Affinity Maturation of Phage Display Antibody Populations using Ribosome Display

            Maria A. Groves and Adrian A. Nickson

 

11. Evolution of Protein Stability Using Ribosome Display

            Andrew Buchanan

 

12. Selection of Lead Antibodies from Naive Ribosome Display Antibody Libraries

            Peter Ravn

 

13. Evolution of Disulfide-Rich Peptide Aptamers Using cDNA Display

            Yuki Mochizuki and Naoto Nemoto

 

14. Peptide Screening Using PURE Ribosome Display

            Hiroyuki Ohashi, Takashi Kanamori, Eriko Osada, Bintang K. Akbar, and Takuya Ueda

 

15. Rapid Selection of High Affinity Binders Using Ribosome Display

            Birgit Dreier and Andreas Plückthun

 

16. mRNA Display-Based Selections Using Synthetic Peptide and Natural Protein Libraries

            Steve W. Cotten, Jianwei Zou, Rong Wang, Bao-cheng Huang, and Rihe Liu

 

17. Identification of Candidate Vaccine Genes Using Ribosome Display

            Liancheng Lei

 

18. Ribosome Display for the Selection of Sac7d Scaffolds

            Barbara Mouratou, Ghislaine Béhar, Lauranne Paillard-Laurance, Stéphane Colinet, and Frédéric Pecorari

 

Part V: Incorporation of Non-Natural Amino Acids for Selection by Ribosome Display and Related Methods

 

19. Charging of tRNAs Using Ribozymes and Selection of Cyclic Peptides Containing Thioethers

            Patrick C. Reid, Yuki Goto, Takayuki Katoh, and Hiroaki Suga

 

20. Update on Pure Translation Display with Unnatural Amino Acid Incorporation

            R. Edward Watts and Anthony C. Forster

 

21. In Vitro Selection of Unnatural Cyclic Peptide Libraries Via mRNA Display

            Zhong Ma and Matthew C.T. Hartman

 

Part VI: Case Studies

 

22. Optimization of CAT-354, a Therapeutic Antibody Directed Against Interleukin-13, Using Ribosome Display

            George Thom and Ralph Minter

 

23. Affinity Maturation and Functional Dissection of a Humanized Anti-RAGE Monoclonal Antibody by Ribosome Display

            Simon E. Hufton

Blurb

Display technologies have become a very powerful way of generating therapeutic lead molecules and specific reagents for increasing our understanding of biology; however, despite being first described shortly after phage display, the use of ribosome display and related methods have been much less widespread.  Since this is in part due to the complexity of the methods, Ribosome Display and Related Technologies: Methods and Protocols seeks to extend their use by collecting expert contributions describing these detailed protocols.  The protocols described range from well-established methods that have been used for a decade to generate high affinity antibodies, which are already in the clinic, to methods that are in their early stages of application such as display of peptides incorporating non-canonical amino acids.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Invaluable and easy to use, Ribosome Display and Related Technologies: Methods and Protocols will be of great benefit to those with general molecular biology or protein engineering experience who wish to select peptides or proteins by display, those with phage display experience who would benefit from the application of ribosome display, as well as those with some ribosome display experience who would like to expand the range of applications to which they are applying the technology.