Ribonuclease P

ISBN-13: 9781461425731 / Angielski / Miękka / 2012 / 283 str.

Fenyong Liu; Sidney Altman

Opis

The Discovery of Ribonuclease P and Enzymatic Activity of Its RNA Subunit Sydney Brenner and Francis H. C. Crick had a specific project in mind when they offered Sidney Altman a position in their group in 1969 to conduct postdoctoral research at the Medical Research Council Laboratory of Molecular Biology (LMB) in Cambridge, England. At the time, an intense international competition was on- ing in as many as a dozen labs to determine the three-dimensional structure of tRNA. At the LMB, Aaron Klug was attacking the structure by crystallographic analysis with Brian F. C. Clark providing large amounts of purified phenylalanine tRNA. (Eventually, Aaron announced his empirically determined 3-D structure of yeast phenylalanine tRNA, a structure that is generally common to tRNAs, due in part to several conserved, novel three-way nucleotide interactions. ) Concurrently, Michael Levitt, a Ph. D. student of Francis, was visually scrutinizing the cloverleaf secondary structure of the 14 tRNA sequences known at the time. Levitt was searching for nucleotide covariation in different parts of the molecules that were conserved in the 14 sequences known at the time. He identified a possible covariation of an apparent Watson-Crick pairing type between the residues at position 15 from the 5' end of the tRNA and residue 48. This association implied these parts of the tRNA, namely the D loop containing residue 15 and the 5' end of the T stem-adjoining residue 48, folded on one another in a tertiary structure shared by different tRNAs.

Spis treści

History of RNase P and overview of its catalytic activity Sidney Altman The Evolution of RNase P and its RNA Jim Brown Structure Modeling of RNase P Eric Westhof Structural studies of RNase P Alfonso Mondragon Folding of bacterial RNase P RNA Tao Pan Kinetic Mechanism of Bacterial RNase P Carol Fierke Roles of metal ions in RNase P catalysis Leif Kirsebom Challenges in RNase P Substrate Recognition: Considering the Biological Context Michael Harris Archaeal RNase P: A Mosaic Of Its Bacterial And Eukaryal Relatives Venkat Gopalan Eukaryote RNase P and RNase MRP David Engelke RNase P From Organelles Agustin Vioque Human RNase P and transcription Nayef Jarrous RNase P as a drug target Roland Hartmann Ribonuclease P as a Tool Fenyong Liu

Blurb

Ribonuclease P (RNaseP), a ribonucleoprotein, is an essential tRNA processing enzyme found in all living organisms. Since its discovery almost 40 years ago, research on RNase P has led to the discovery of the catalytic properties of RNA, and of the only known, naturally occurring RNA enzymes, RNase P catalytic RNA. The description of the catalytic properties of RNA has provided fundamental insight into the RNA world and these catalytic properties are being harnessed as therapeutic and prevention strategies for acquired and inherited diseases. Ribonuclease P is the first book to provide a comprehensive collection covering all aspects of current research on RNase P. The topics include kinetic and structural analysis, mechanism of catalysis, and its regulation and biogenesis in prokaryotes, eukaryotes, and organelles. Furthermore, research progresses on developing RNase P as a potential drug target for antimicrobial development and as a gene-targeting tool for anti-infective and anticancer therapy are also included. This book should be of general interests to molecular biologists and biochemists in both the academic section and pharmaceutical industry.