Recombinant Proteins from Plants: Methods and Protocols

ISBN-13: 9781493950041 / Angielski / Miękka / 2016 / 296 str.

Jacqueline MacDonald; Igor Kolotilin; Rima Menassa

Spis treści

Part I: Vectors and Transformation

 

1. Chloroplast Based Expression of Recombinant Proteins by Gateway® Cloning Technology

Johanna Gottschamel and Andreas Lössl                                                                              

 

2.  Multigene Engineering in Rice Using High-Capacity Agrobacterium Tumefaciens BIBAC Vectors

Ruifeng He

 

3. Virus-Derived Vectors for the Expression of Multiple Proteins in Plants

Pooja Saxena, Eva C. Thuenemann, Frank Sainsbury, and George P. Lomonossoff

 

4. Transient Protein Expression by Agroinfiltration In Lettuce

Qiang Chen, Matthew Dent, Jonathan Hurtado, Jake Stahnke, Alyssa Mcnulty, Kahlin Leuzinger, Huafang Lai

 

 

5. Production of Recombinant Proteins in the Chloroplast of the Green Alga Chlamydomonas Reinhardtii          

Daniel Guzmán-Zapata, Karla Soledad Macedo-Osorio, Alma Lorena

Almaraz-Delgado, Noé Durán-Figueroa, and Jesus Agustín Badillo-Corona

 

Part II: Product Improvements

 

6.  Efficient, Antibiotic Marker-Free Transformation of a Dicot and a Monocot Crop with Glutamate 1-Semialdehyde Aminotransferase Selectable Marker Genes

Nicoletta Ferradini, Angelica Giancaspro, Alessandro Nicolia, Agata Gadaleta, Fabio Veronesi, Daniele Rosellini

 

7. Transient Expression of Mammalian Genes In Nicotiana Benthamiana To Modulate N-Glycosylation  

Alexandra Castilho

and Herta Steinkellner

 

8. Companion Protease Inhibitors for the In Situ Protection of Recombinant Proteins in Plants

Stéphanie Robert, Philippe V. Jutras, Moustafa Khalf, Marc-André D’Aoust, Marie-Claire Goulet, Frank Sainsbury and Dominique Michaud 

 

Part III: Extraction and Scale-Up

 

9. Production of Recombinant Cholera Toxin B Subunit in Nicotiana Benthamiana Using GENEWARE® Tobacco Mosaic Virus Vector

Lauren Moore, Krystal Hamorsky, and Nobuyuki

Matoba

 

10. Total Soluble Protein Extraction for Improved Proteomic Analysis Of Transgenic Plant Roots 

Manish L. Raorane, Joan O. Narciso, and Ajay Kohli

 

11. Temporary Immersion Bioreactors for the Contained Production Of Recombinant Proteins In Transplastomic Plants

Sherwin Barretto, Franck Michoux, and Peter J. Nixon

 

12. Plant Cell-Based Recombinant Antibody Manufacturing with A 200 L Orbitally Shaken Disposable Bioreactor                                                                                                                                                

Nicole Raven, Stefan Schillberg, and Stefan Rasche

 

13. Liquid-Liquid Phase Separation of Oil Bodies From Seeds                                                                      

Cory L. Nykiforuk

 

14. Continuous Flow Separation of Hydrophobin Fusion Proteins from Plant Cell Culture

Extract              

Lauri J. Reuter, Andrew J. Conley, and Jussi J. Joensuu

 

Part IV: Analysis

 

15. Molecular Analyses of Transgenic Plants

Kurniawan Rudi Trijatmiko, Felichi Mae Arines, Norman Oliva, Inez Slamet-Loedin, and Ajay Kohli

 

16. Analysis of Recombinant Proteins In Transgenic Rice Seeds: Identity, Localization, Tolerance To Digestion, And Plant Stress Response

Yuhya Wakasa and Fumio Takaiwa

 

17. Real-Time PCR-Based Quantitation Method for The Genetically Modified Soybean Line GTS 40-3-2

Kazumi Kitta, Reona Takabatake, and Junichi Mano

 

18. A Standardized Lepidopteran Bioassay to Investigate the Bioactivity Of Insecticidal Proteins Produced In Transgenic Crops

Gerson Graser and Frederick S. Walters

 

Part V: Antibodies for Pathogen Resistance

 

19. Generating Transgenic Plants

Showing Antibody-Mediated Pathogen Resistance

Dieter Peschen, Stefan Schillberg, and Rainer Fischer

 

 

Blurb

This volume provides up-to-date scientific achievements from the world’s top researchers. Recombinant Proteins from Plants: Methods and Protocols, Second Edition guides readers through protocolsfor use with a variety of plant expression systems. Various aspects of production are covered including vector selection and cloning; product improvements for stability, glycosylation, and antibiotic-free selection; extraction and scale-up; and analysis of transgenic plants and their recombinant proteins. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Recombinant Proteins from Plants: Methods and Protocols, Second Edition is an ideal reference for those who are interested in plant molecular biology and molecular farming.