Recombinant Glycoproteins: Methods and Protocols

ISBN-13: 9781071636657 / Angielski

Steven B. Bradfute

Spis treści

Part I: Viral Surface Glycoproteins

 

1. Production and Purification of Hantavirus Glycoproteins in Drosophila melanogaster S2 Cells

            Annalisa Meola and Pablo Guardado-Calvo

 

2. Production and Purification of Filovirus Glycoproteins

            Madeleine Noonan-Shueh, M. Javad Aman, and Shweta Kailasan

 

3. Modification of N-Linked Glycan Sites in Viral Glycoproteins

            Nicholas J. Lennemann, Lochlain Corliss, and Wendy Maury

 

4. Production of Influenza Virus Glycoproteins Using Insect Cells

            Madhumathi Loganathan, Benjamin Francis, and Florian Krammer

 

5. SARS-CoV-2 S-Protein – Ace2 Binding Analysis Using Surface Plasmon Resonance

            Jason Baardsnes and Béatrice Paul-Roc

 

6. A Biosensor Assay Based on Coiled-Coil-Mediated Human ACE2 Receptor Capture for the Analysis of Its Interactions with the SARS-CoV-2 Receptor Binding Domain

            Catherine Forest-Nault, Izel Koyuturk, Jimmy Gaudreault, Alex Pelletier, Denis L’Abbé, Brian Cass, Louis Bisson, Alina Burlacu, Laurence Delafosse, Matthew Stuible, Olivier Henry, Gregory De Crescenzo, and Yves Durocher

 

Part II: Protozoal and Fungal Glycoproteins and Polysaccharide Conjugates

 

7. Production and Purification of Plasmodium Circumsporozoite Protein in Lactococcus lactis

            Mohammad Naghizadeh, Susheel K. Singh, Jordan Plieskatt, Ebenezer Addo Ofori, and Michael Theisen

 

8. Analysis of Caenorhabditis Protein Glycosylation

            Katharina Paschinger, Jorick Vanbeselaere, and Iain B.H. Wilson

 

9. Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates

            Federico Urbano-Munoz, Caitlyn E. Orne, Mary N. Burtnick, and Paul J. Brett

 

Part III: Mammalian Glycoproteins

 

10. Overexpression and Purification of Mitogenic and Metabolic Fibroblast Growth Factors

            Phuc Phan, Shivakumar Sonnaila, Gaetane Ternier, Oshadi Edirisinghe, Patience Salvalina Okoto, and Thallapuranam Krishnaswamy Suresh Kumar

 

11. Production and Purification of Antibodies in Chinese Hamster Ovary Cells

            Lauren Stuart

 

12. Mammalian Antigen Display for Pandemic Countermeasures

            Andrea Quezada, Ankur Annapareddy, Kamyab Javanmardi, John Cooper, and Ilya J. Finkelstein

 

Part IV: Analysis of Glycoproteins

 

13. Analysis of Native and Permethylated N-Glycan Isomers Using MGC-LC-MS Techniques

            Andrew I. Bennett, Oluwatosin Daramola, Md Mostofa Al Amin Bhuiyan, Vishal Sandilya, and Yehia Mechref

 

14. Targeted Glycoproteomics Analysis Using MRM/PRM Approaches

            Cristian D. Gutierrez Reyes, Akeem Sanni, Moyinoluwa Adeniyi, Damir Mogut, Hector R. Najera Gonzalez, Parisa Ahmadi, Mojgan Atashi, Sherifdeen Onigbinde, and Yehia Mechref

 

15. Targeted Analysis of Permethylated N-Glycans Using MRM/PRM Approaches

            Cristian D. Gutierrez Reyes, Akeem Sanni, Damir Mogut, Moyinoluwa Adeniyi, Parisa Ahmadi, Mojgan Atashi, Sherifdeen Onigbinde, and Yehia Mechref

 

16. Hydrophilic Interaction Liquid Chromatography (HILIC) Enrichment of Glycopeptides Using PolyHYDROXYETHYL A

            Mona Goli, Peilin Jiang, Mojibola Fowowe, Md Abdul Hakim, and Yehia Mechref

 

17. O-Glycoproteomics Sample Preparation and Analysis Using NanoHPLC and Tandem MS

            Junyao Wang, Sherifdeen Onigbinde, Waziha Purba, Judith Nwaiwu, and Yehia Mechref

 

Part V: Considerations and Alternatives for Glycoprotein Production and Purification

 

18. Solubilization of Oligomeric Cell-Free Synthesized Proteins Using SMA Copolymers

            Jessica Ullrich, Lisa Haueis, Carsten Ohlhoff, Anne Zemella, Stefan Kubick, and Marlitt Stech

 

19. Cell-Free Systems for the Production of Glycoproteins

            Erik Bidstrup, Yong Hyun Kwon, Keehun Kim, Chandra Kanth Bandi, Rochelle Aw, Michael C. Jewett, and Matthew P. DeLisa

 

20. Considerations for Glycoprotein Production

            Elizabeth C. Clarke

Blurb

This detailed volume explores the production and purification of glycoproteins for therapeutic use or basic research, as well as the necessary in-depth understanding of cellular and acellular systems available for these purposes, their advantages and disadvantages, and considerations for choosing the most appropriate system for the desired application. Beginning with chapters on viral-encoded glycoproteins and those found in other pathogens, the book continues by examining the production of mammalian glycoproteins, the analysis of glycoprotein content, cell-free synthesis of glycoproteins and considerations for production and purification of glycoproteins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to the respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Recombinant Glycoproteins: Methods and Protocols provides a wide range of guidelines for studying these vitally important proteins.