RNA Nanotechnology and Therapeutics: Methods and Protocols

ISBN-13: 9781493925612 / Angielski / Twarda / 2015 / 239 str.

Peixuan Guo; Farzin Haque

Opis

This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls.Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.

Spis treści

1. Overview of Methods in RNA Nanotechnology - Synthesis, Purification and Characterization of RNA Nanoparticles
Farzin Haque and Peixuan Guo

2. Multiple Approaches for the Investigation of Bacterial Small Regulatory RNAs Self-Assembly                 
Christophe Lavelle, Florent Busi, and Véronique Arluison

3. Measuring the Elasticity of Ribonucleotide(s)-Containing DNA Molecules Using AFM
Kyung Duk Koh, Hsiang-Chih Chiu, Elisa Riedo, and Francesca Storici

4. Silver Nanoclusters for RNA Nanotechnology: Steps Towards Visualization and Tracking of RNA Nanoparticle Assemblies
Kirill A. Afonin, Danielle Schultz, Luc Jaeger, Elisabeth Gwinn, and Bruce A. Shapiro

5. Purification of RNA Nanoparticles by Ultracentrifugation
Daniel L. Jasinski, Farzin Haque, and Peixuan Guo

6. HPLC Purification of RNA Aptamers up to 59 Nucleotides with Single Nucleotide Resolution
Zhen Huang, Chi-yen Lin, William Jaremko, and Li Niu

7. RNA Nanoparticles with Thermostable Motifs and Fluorogenic Modules for Real-Time Detection of RNA Folding and Turnover In Vivo
Hui Zhang, Fengmei Pi, Dan Shu, Mario Vieweger, and Peixuan Guo

8. Fluorescence Labeling of Short RNA by Oxidation at the 3’-End
Chen Qiu, Wang-Yi Liu, and Yong-Zhen Xu

9. Methods and Assays for Specific Targeting and Delivery of RNA Nanoparticles to Cancer Metastases
Piotr Rychahou, Yi Shu, Farzin Haque, Jiyao Hu,  Peixuan Guo, and B. Mark Evers

10. Functional Assays for Specific Targeting and Delivery of RNA Nanoparticles to Brain Tumor
Tae Jin Lee, Farzin Haque, Mario Vieweger, Ji Young Yoo, Balveen Kaur, Peixuan Guo, and Carlo M. Croce

11. Aptamer-Mediated Nanoparticle Interactions: From Oligonucleotide-Protein Complexes to SELEX   Screens
Laetitia Evadé, Eric Dausse, Said Taouji, Emilie Daguerre, Eric Chevet, and Jean-Jacques Toulmé

12. Methods for Assembling B-Cell Lymphoma Specific and Internalizing Aptamer-siRNA Nanoparticles Via the Sticky Bridge
Jiehua Zhou, John J. Rossi, and Ka To Shum

13. A High Throughput Screening Assay for the Functional Delivery of Splice-Switching Oligonucleotides in Human Melanoma Cells
John M. Dean and Robert K. DeLong

14. Design, Assembly, and Evaluation of RNA-Protein Nanostructures
Hirohisa Ohno, Eriko Osada, and Hirohide Saito

15. Mapping RNA Interactions to Proteins in Virions Using CLIP-Seq
Baochang Fan, Peng Ni, and C. Cheng Kao

16. Mapping Protein-RNA Interactions by RCAP, RNA-Crosslinking and Peptide Fingerprinting
Robert Vaughan and C. Cheng Kao

O autorze

Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) (859)218-0128 (Cell Phone): (513)728-1411 peixuan.guo@uky.edu (future)