1 Electrophoretic Analysis of Reversible Interactions 2 Determination of Affinity Constants by Capillary Electrophoresis 3 Measurement of Binding Constants by Frontal Affinity Chromatography 4 Determination of Affinity Constants by Partition Equilibrium Methods 5 Determination of Transmembrane Protein Affinities for Solutes by Frontal Chromatography 6 Modeling of Chiral Liquid Chromatography using an Immobilized Protein as the Selector 7 Determination of Receptor-Ligand Affinity Constants 8 Determination of Equilibrium and Kinetic Constants for the Interaction of GTPases with Nucleotides, Regulators and Effectors 9 Biospecific Interaction Analysis using Integrated Optics Techniques 10 Receptor Binding Studies on Lipid Vesicles using the BIAcore 11 The Analysis of Surface Plasmon Resonance-based Biosensor Data using Numerical Integration: The Epidermal Growth Factor Receptor-Ligand Interaction as an Example 12 Affinity Chromatography for the Determination of Interactions between Drugs and Proteins: HPLC and CE Methods Employing ‘Secondary and Dynamic’ Equilibria
Per Lundahl, Andreas Lundqvist, Eva Greijer- all Department of Biochemistry, Biomedical Center, Uppsala University, Uppsala, Sweden