Purine Metabolism in Man-IV: Part B: Biochemical, Immunological, and Cancer Research

ISBN-13: 9781475703924 / Angielski / Miękka / 2012 / 520 str.

Chris H. De Bruyn

Opis

These two volumes, entitled "Purine Metabolism in Man IV" con- tain the papers presented at the Fourth International Symposium on Human Purine and Pyrimidine Metabolism," held in Maastricht (The Netherlands), June 1982. The proceedings of the three previous meet- ings in Tel Aviv (Israel, 1973), Baden (Austria, 1976) and Madrid (Spain, 1979) were also published by Plenum Press. In the past few years interest in purine and pyrimidine metabo- lism under normal and pathological conditions has been growing rapid- ly. Apart from the more or less classical topics such as hyperuricae- mia, clinical gout and urolithiasis, an increasing number of papers relating to other fields have been presented at successive meetings. Knowledge derived from the study of purine metabolism in relation to lymphocyte function, for instance, has opened up new possibilities for immunomodulation and leukaemia chemotherapy, with eventual conse- quences for other types of cancer. At previous meetings there have been pointers implicating purine metabolism in relation to normal cardiac and skeletal muscle function. During the present meeting mych new data on both issues have been re- ported which indicate clear differences in the pathways of ATP metabo- lism. The widening of the field of interest is also illustrated by the recent work on infectious disease: exploitation of the differences in purine metabolic pathways in certain parasites compared with those in human cells has resulted in new rationales for therapy being devel- oped.

Spis treści

of Part B.- I. Biochemical and Enzymological Studies.- Evidence of a New Type of Phosphoribosylpyrophosphate Synthetase with Abnormal Kinetic Properties in Hyperuricemias.- Radioimmunoassay Studies of Human Phosphoribosylpyrophosphate Synthetase.- Synthesis of (14O-Ribose-5-Phosphate and (14C)-Phosphoribosylpyrophosphate and Their Use in New Enzyme Assays.- Purine Synthesis during Amino Acid Starvation of Lympho-blasts with HPRT Deficiency of PP-Ribose-P Synthetase Overactivity Decreases Less than in Normal Cells.- Purine Synthesis and Salvage in Brain and Liver.- Kinetic Studies of Hypoxanthine-Guanine Phosphoribosyl-transferase in Intact Cells.- Hypoxanthine-Guanine Phosphoribosyltransferase in Human Lymphoblastoid Cells: Confirmation of Four Structural Variants and Demonstration of a New Variant (HPRTAnn Arbor).- The Primary Structure and Posttranslational Modification of Human Hypoxanthine-Guanine Phosphoribosyl-transferase.- Studies of the Catalytically-Active Form of Hypoxanthine-Guanine Phosphoribosyltransferase from Yeast.- Human Lymphocytic Ecto-5?-NT: Its Determination and Partial Characterization.- Distribution of Ecto-5?-Nucleotidase in Plasmamembrane Subfractions of Human Lymphoblastoid Cells.- AMP Deaminase Isozymes in Human Blood Cells.- Immunohistochemical Localization of Adenosine Deaminase in Rat and Calf Tissues.- Characterization of Adenosine Deaminating Activity in Normal and Adenosine Deaminase Deficient Human Tissue.- S-Adenosylhomocysteine Hydrolase Activity in Defects of Enzymes of Purine Metabolism and Inhibition by Various Purine Compounds.- Deoxyguanosine Kinase from Human Placenta.- Phosphorylation of Deoxyguanosine in Rat Liver Mitochondria.- 5?-Methylthioadenosine is the Major Source of Adenine in Human Cells.- Determination of a Histidine Residue at the Yeast Orotate Phosphoribosyltransferase Active Site.- II. Purine Metabolism in Normal Lymphoid Cells.- Changes in Purine Salvage Pathway Enzyme Activities During Human Lymphocyte Differentiation Induced by Thymosin Fraction 5.- Purine Metabolism in Human Thymocyte Subsets: Relevance for Lymphocytic Differentiation.- Purine Metabolism in Splenocytes and Thymocytes of Various Mammalian Species.- Purine Interconversion Pathways in T, B, T? and T-T? Cells from Human Peripheral Blood.- Metabolism of Guanosine and Deoxyguanosine in Mammalian Lymphocytes and Their Influence on Mitogenic Stimulation.- Concentration of Nucleotides in Peripheral Blood Lymphocytes of Various Mammalian Species.- Plasma Membrane 5?-Nucleotidase and Other Purine Enzymes in Murine Lymphocytes.- III. Purine Metabolism in Relation to Lymphocyte Function.- Biochemical Basis for Lymphocyte Dysfunction in Adenosine Deaminase and Purine Nucleoside Phosphorylase Deficiencies.- Absence of Lymphocyte Ecto-5?-Nucleotidase in Infants with Reticuloendotheliosis and Immunodeficiency.- Regulation of Human Lymphoblast Ecto-5?-Nucleotidase by Zinc.- Decreased T Cell Ecto-5?-Nucleotidase Activity in Agammaglobulinemia Patients is Not Due to Altered Proportions of Helper and Suppressor T Cells as Defined by the Monoclonal Antibodies OKT4 and OKT8.- Metabolic Findings in a Patient with Adenosine Deaminase Deficiency and Severe Combined Immunodeficiency.- Purine Metabolism in Intact Cells from a Purine Nucleoside Phosphorylase Deficient Child.- Inosine Formation from Hypoxanthine by Intact Erythrocytes and Fibroblasts of an Immunodeficient Child with Purine Nucleoside Phosphorylase Deficiency.- Mononuclear Cells in S-Phase in a Patient with Purine Nucleoside Phosphorylase Deficiency.- Involvement of Dietary Nucleotides in T Lymphocyte Function.- Nucleotide Levels in Peripheral Blood Mononuclear Cells of Immunodeficient Children: Problems of Measurement.- IV. Immunopharmacology.- Inhibition of Murine Suppressor T Cell Development by 2?-Deoxyguanosine in Vivo.- Biochemical and Immunological Evaluation of Long Term Coformycin Administration in the Mouse.- Increased Incidence of Homogeneous Immunoglobulins in Irradiated, Reconstituted Mice after Prolonged Treatment with 2?-Deoxyguanosine.- S-Adenosylhomocysteine Hydrolase as a Pharmacological Target for the Inhibition of Transmethylation.- Selective Inhibition of Various Mitogen Responses in Human Lymphocytes.- Sensitivity of Human T and B Lymphoblasts to Immuno-regulatory Drugs.- Inhibition of in Vitro Lymphocyte Proliferation and B Cell Differentiation by Deoxyguanosine: Evidence for Separate Mechanisms.- V. Purine Metabolism in Macrophages.- Effect of Purine Nucleosides on Rat Macrophage Function.- Purine Metabolism in Rat Macrophages.- VI. Enzymes of Purine Metabolism as Markers for Malignancy.- Purine Metabolizing Enzyme Activities in Lymphoblastic Leukemia.- Purine and Pyrimidine Enzyme Markers in Human Lymphoid Malignancies.- Ecto-5?-Nucleotidase as a Leukemia Marker.- Immunohistochemical Localization of Adenosine Deaminase in Human Lymphoid Tissues and Lymphomas.- 5?Nucleotidase and Adenosine Deaminase Activities in Human Lymphocytes and Lymphoblastoid Cell Lines.- Properties and Activities of Phosphoribomutase in Human Leukemic Cells.- Uptake of AMP into K 562 and HL-60 Leukemic Cell Lines.- 5?-Methylthioadenosine Phosphorylase Deficiency in Malignant Cells: Recessive Expression of the Defective Phenotype in Intraspecies (Mouse x Mouse) Hybrids.- The Activity of Adenosine Desaminase in Serum and Leukocytes of Leukemic Patients.- Uptake of Thymidine by Plasma Membrane Vesicles of L5178Y Murine Leukemic Cells.- Adenosine Deaminase Isozymes in Transformed Cells.- Adenosine Deaminase (ADA; E.C. no. 3.5.4.4.) in Colorectal Adenocarcinoma in Man.- VII. Nucleoside Metabolism and the Development of Anti-Leukemic Agents.- Biochemical Basis for the Differential Effects of Deoxyco-formycin on Human Leukemias.- Sensitivity of T-Leukemic Cells to Deoxyguanosine and Arabinosyl Guanine.- Exploiting Biochemical Differences in Leukemia.- Effects of 3-Deazaguanosine and 3-Deazaguanine on the Growth and Maturation of the Human Promyelo-cytic Leukemia Cell Line, HL-60.- Intralymphocytic Adenosine Deaminase (ADA) and Purine Nucleoside Phosphorylase (PNPase) Activity in Chronic Lymphocytic Leukemia (CLL): Effect of Thymostimulin.- Deoxyadenosine Induced G1 Phase Arrest in Leukemic T Cells.- Poly(A)+RNA as a Possible Target for Deoxyadenosine Induced G1/G0 Lymphotoxicity.- Modulation of Arabinosyladenine Metabolism by 2?-Deoxy-coformycin in the Therapy of Human Acute Leukemia.- Cell Cycle Independent Lymphocytotoxicity of 2-Chloro-deoxyadenosine.- Ecto-Adenosine Triphosphatase: An Enzyme Correlating with Resistance to Deoxynucleoside Growth Inhibition.- Purine Deoxyribonucleoside Induced Hepatotoxicity in the Mouse.- VIII. Anti-Cancer Agents.- 6-Mercaptopurine: Pharmacokinetics in Animals and Preliminary Results in Children.- Enzyme Inhibition by Phosphonate Analogues of dTTP.- Inhibition of Thymidylate Synthetase by the New Quinazoline Antifolate, CB 3717; Enzyme Purification and Kinetics.- Plasma Nucleoside and Base Levels Following Treatment with the New Thymidylate Synthetase Inhibitor CB 3717.- Influence of Cytostatics on ATP-Levels of Leukemic Cells.- Thymidine Sensitivity and Deoxynucleotide Pools of Human Lymphoid and Melanoma Cells in Vitro.- High Uridine Catabolic Activity in Cultured Human Melanoma Cells.- Synergism between Purines and Thymidine (TDR) in Reversal of Methotrexate (MTX) Toxicity in Mice Using Continuous Infusions.- Cell-Cycle Dependent Variation in the Levels of Deoxy-ribonucleoside Triphosphate in Mouse T-Lymphoma Cells.- Tumor Specific Conversion of a Pyrimidine Antimetabolite.- IV. Purine Metabolism in Skeletal Muscle.- Isotachophoretic Analysis of Some Compounds Involved in Energy Metabolism in Normal and Pathological Human Muscle Extracts.- Adenine Metabolism in Rat Skeletal Muscle.- Purine Nucleotide Metabolism in Primary Rat Skeletal Muscle Cultures.- Metabolism of AMP in Muscle Extracts from Patients with Deficient Activity of Myoadenylate Deaminase.- Effect of Hypoxia and Exercise on Nucleotide Metabolism in Man.- Malignant Hyperthermia: Adenine Incorporation and Adenine Metabolism in Human Platelets, Influenced by Halothane.- Enzymes of Purine Metabolism in Muscle Specimens from Patients with Duchenne-Type Muscular Dystrophy.- X-Linked Recessive (Duchenne) Muscular Dystrophy (DMD) and Purine Metabolism: Effects of Oral Allopurinol and Adenylate.- Non-Specific Adenylate Deaminase from Snail Foot Muscle.- Potential Role of Non-Specific Snail Muscle Adenylate Deaminase in Operating of NADH-Dependent Dehydrogenases.- X. Purine Metabolism in Cardiac Muscle.- Adenine Nucleotide Biosynthesis in Cardiac Muscle: Regulation and Intervention.- Ribose Prevents the Propranolol-Induced Reduction of Myocardial Adenine Nucleotide Biosynthesis.- Balance of Purine Nucleotides and Catabolites in the Isolated Ischemic Rat Heart.- Inosine Incorporation into Adenine- and Guanine Nucleotides in the Isolated Perfused Rat Heart is Stimulated after Ischemia.- The Ca-Antagonist Nifedipine Reduces Purine Nucleoside and Oxypurine Release from Ischemic Heart.- Combined Use of Radioenzymatic Assay and High Pressure Liquid Chromatography for the Detection of Myocardial Xanthine Oxidase/Dehydrogenase.- Purification and Characterization of Pig Heart AMP-Deaminase.- AMP Deaminase from Necrotic Rat Heart.