Proteome and Protein Analysis

ISBN-13: 9783642640605 / Angielski / Miękka / 2012 / 372 str.

R. M. Kamp; D. Kyriakidis; T. Choli-Papadopoulou

Opis

The papers selected here cover new, sensitive and rapid methods for the analysis of proteins, with special emphasis on the proteome. In addition to the experimental details, the advantages and limitations of the methodological approaches are discussed, and topics include sequencing analysis, sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and folding, functional implications of domains and newly emerging investigative methods, allowing analysis of the expression of genes.

Spis treści

I Proteomics and Mass Spectrometry.- 1 Strategies and Methods for Proteome Analysis.- 2 Protein Sequencing or Genome Sequencing. Where Does Mass Spectrometry Fit into the Picture?.- 3 Reversible Negative Staining of Protein on Electrophoresis Gels by Imidazole-Zinc Salts: Micropreparative Applications to Proteome Analysis by Mass Spectrometry.- 4 Direct Analysis of Protein Complexes.- 5 De novo Sequencing of Proteins With Mass Spectrometry Using the Differential Scanning Technique.- 6 Characterization Of Gel Separated Proteins.- II Structure Analysis.- 7 Protein Structure and Dynamics by NMR in Solution.- 8 Elucidation of Functionally Significant Structural Modifications by Matrix-Assisted Laser Deorption/Ionization Time-of-Flight Mass Spectrometry with Post-Source Decay Analysis.- 9 Detection of Specific Zinc Finger Peptide Complexes with Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry.- 10 Epitode Mapping for the Monoclonal Antibody that Inhibits Intramolecular Flavin to Heme Electron Transfer in Flavocytochrome B2 from Baker’s Yeast (L-Lactate Dehydrogenase).- 11 A MALDI-TOF Mass Spectrometry Approach to Investigate the Defense Reactions in Drosophila Melanogaster, an Insect Model for the Study of Innate Immunity.- 12 A New Method for the Isolation of the C-Terminal Peptide of Proteins by the Combination of Selective Blocking of Proteolytic Peptides and Cation Exchange Chromatography.- 13 The Folding Pathway of Disulfide Containing Proteins.- 14 Cavity Supported HPLC of cis/trans Isomers of Proline Containing Peptides Using Cyclodextrins and Calixarenes.- 15 The Perception of Hydrophobic Clusters in the Native and Partially Unfolded States of a Protein.- 16 Structure, Dynamics and Function of the Proton Pump Bacteriorhodopsin.- 17 Structural Characterization of Porcine Seminal Plasma Psp-I/Psp-li, A Paradigm Spermadhesin.- 18 Expression and Characterization of Saposin-Like-Proteins.- III Protein-Protein and Protein-DNA Interaction.- 19 A Structural Model for the P53 Complex with DNA Response Elements: Implications for P53 Function and Future Research Directions.- 20 Overproduction, Purification and Structural Studies on the Zn Containing S14 Ribosomal Protein from Thermus thermophilus.- 21 Bimolecular Interaction of Matrix Metalloproteinases and Their Inhibitors TIMPs.- IV Posttranslational Modifications.- 22 Enzymatic and Chemical Deblocking of N-Terminally Modified Proteins.- 23 Exploring Functions of Glycosylation in Host Defence Using Novel Oligosaccharide Sequencing Technology.- 24 Proteoglycans: Biological Roles and Strategies for Isolation and Determination of their Glycan Constituents.

Blurb

Selected papers presented at the MPSA 98 are covering new, sensitive and rapid methods for the analysis of proteins, with special emphasis on the total cell proteins, the proteome. In addition to the experimental details, the advantages and limitations of the methodological approaches are discussed.
Topics included are: Protein sequencing analysis, protein and peptide sample preparation, mass spectrometry, NMR, analysis of post-translational modifications, purification of recombinant proteins, protein-protein and protein-DNA interactions, structure prediction, modeling and protein folding, functional implications of protein domains and newly emerging methods for the investigation of the proteome, allowing to analyse the expression of genes