Protein'ligand Interactions: Methods and Applications

ISBN-13: 9781588293725 / Angielski / Twarda / 2005 / 568 str.

G. Ulrich Nienhaus; G. Ulrich Nienhaus

Opis

A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls."

Spis treści

Isothermal Titration Calorimetry Edwin A. Lewis and Kenneth P. Murphy Direct Optical Detection of Protein-Ligand Interactions Frank Gesellchen, Bastian Zimmermann, and Friedrich W. Herberg Label-Free Detection of Protein-Ligand Interactions by the Quartz Crystal Microbalance Andreas Janshoff and Claudia Steinem Measurement of Solvent Accessibility at Protein-Protein Interfaces Jeffrey G. Mandell, Abel Baerga-Ortiz, Arnold M. Falick, and Elizabeth A. Komives Hydrophobic Interaction Chromatography: Harnessing Multivalent Protein-Surface Interactions for Purification Procedures Herbert P. Jennissen Sedimentation Velocity Method in the Analytical Ultracentrifuge for the Study of Protein-Protein Interactions Claus Urbanke, Gregor Witte, and Ute Curth Protein-Ligand Interaction Probed by Time-Resolved Crystallography Marius Schmidt, Hyotcherl Ihee, Reinhard Pahl, and Vukica Srajer X-Ray Crystallography of Protein-Ligand Interactions Ilme Schlichting Combined Use of XAFS and Crystallography for Studying Protein-Ligand Interactions in Metalloproteins Richard W. Strange and S. Samar Hasnain NMR Studies of Protein-Ligand Interactions Till Maurer Probing Heme Protein-Ligand Interactions by UV/Visible Absorption Spectroscopy Karin Nienhaus and G. Ulrich Nienhaus Ultrafast Time-Resolved IR Studies of Protein-Ligand Interactions Manho Lim and Philip A. Anfinrud Monitoring Protein-Ligand Interactions by Time-Resolved FTIR Difference Spectroscopy Carsten Kötting and Klaus Gerwert Proteins in Motion: Resonance Raman Spectroscopy as a Probe of Functional Intermediates Uri Samuni and Joel M. Friedman Fluorescence Polarization/Anisotropy Approaches to Study Protein-Ligand Interactions: Effects of Errors and Uncertainties David M. Jameson and GaborMocz Ligand Binding With Stopped-Flow Rapid Mixing Mark S. Hargrove Circular Dichroism Spectroscopy for the Study of Protein-Ligand Interactions Alison Rodger, Rachel Marrington, David Roper, and Stuart Windsor High-Throughput Screening of Interactions Between G Protein-Coupled Receptors and Ligands Using Confocal Optics Microscopy Lenka Zemanová, Andreas Schenk, Martin J. Valler, G. Ulrich Nienhaus, and Ralf Heilker Single-Molecule Study of Protein-Protein and Protein-DNA Interaction Dynamics H. Peter Lu Application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding Theodore L. Hazlett, Qiaoqiao Ruan, and Sergey Y. Tetin Atomic Force Microscopy Measurements of Protein-Ligand Interactions on Living Cells Robert H. Eibl and Vincent T. Moy Computer Simulation of Protein-Ligand Interactions: Challenges and Applications Sergio A. Hassan, Luis Gracia, Geetha Vasudevan, and Peter J. Steinbach Force Probe Molecular Dynamics Simulations Helmut Grubmüller Study of Ligand-Protein Interactions by Means of Density Functional Theory and First-Principles Molecular Dynamics Carme Rovira Index

Blurb

Molecular recognition and binding of ligands (atoms, ions, and molecules) by proteins with high sensitivity and selectivity is of central importance to essentially all biomolecular processes and of key importance for the basic and applied sciences. In Protein-Ligand Interactions: Methods and Applications, leading experts with hands-on experience describe in detail a broad selection of established and emerging techniques for studying the interaction between proteins and ligands, including bulk biochemical techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved X-ray crystallography. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Protein-Ligand Interactions: Methods and Applications offers novice and expert researchers alike a broad selection of powerful and widely applicable techniques that can be used to efficiently and successfully solve the task of characterizing protein-ligand interactions.