Proteinase Inhibitors: Proceedings of the 2nd International Research Conference

ISBN-13: 9783642879685 / Angielski / Miękka / 2012 / 754 str.

H. Fritz; Harald Tschesche; L. J. Greene

Opis

These proceedings contain the papers presented at the Bayer-Symposium V which was held in Grosse Ledder on October 16-21, 1973 and which was simultaneously the Second International Research Conference on Proteinase Inhibitors. The cordial atmosphere, the frank discussions and the profitable exchange of ideas during the First International Research Conference on Proteinase Inhibitors which was held on November 4--6, 1970 in Munich were a stimulant for us to consider holding a Second Conference especially as the steadily increasing research activities in this and related fields called for a new assessment of recent efforts in proteinase inhibitor research. In the three years since the First Conference was held, this field has developed particularly in the areas of molecular aspects of protein-protein interactions and in the extension to inhibitors of nonserine proteinases and peptidases. In planning this meeting the Organizing Committee was faced with the problem of selecting a limited number of participants and we decided that it was appropriate and necessary to focus in detail on the chemistry of proteinase inhibitors, their interac tions with a variety of enzymes and also to invite more general papers to survey the physiological and clinical aspects of the subject which we felt would be of importance in the future."

Spis treści

Proteinase Inhibitors.- A Turbulent Life.- Welcome.- Section I Plasma Proteinase Inhibitors.- Biochemistry of Proteinase Inhibitors from Human Plasma: A Review of Recent Development.- Purification, Properties, and Composition of ?1-Trypsin Inhibitor from Human Plasma.- Properties of Human ?1-Antitrypsin.- Human ?1-Antitrypsin Phenotypes.- Isolation and Fragmentation of ?1-Antitrypsin.- The Interaction of Human Cationic Trypsin and Chymotrypsin II with Human Serum Inhibitors.- Studies on the Quaternary Structure of Human Serum ?2-Macroglobulin.- The Unique Nature of the Interaction of ?2-Macroglobulin with Proteinases.- Biology and Pathology of Plasma Proteinase Inhibitors.- Interaction between Endogenous Proteases and Plasma Protease Inhibitors in vitro and in vivo.- Plasma Clearance of Human Antiproteinase/Proteinase Complexes.- Protease Inhibitors in Human Synovial Fluids of Patients with Joint Diseases.- Proteinase Inhibitors of the Respiratory Tract: Studies on the Structural Relationship between Acid-Stable Inhibitors Present in the Respiratory Tract, Plasma and Urine.- Section II Proteinases and Proteinase Inhibitors in Fertilization.- Biochemistry and Clinical Significance of the Trypsin-Like Proteinase Acrosin from Boar and Human Spermatozoa.- Studies on the Purification and Characterization of Boar Acrosin.- Proteinase Inhibitors and Proteinases of Human Semen.- Characterization of Two Proteinase Inhibitors from Human Seminal Plasma and Spermatozoa.- Isolation of Proteinase Inhibitors from Boar Sperm Acrosomes and Boar Seminal Plasma and Effect on Fertilization.- Purification,Characterization,and Structural Studies of Proteinase Inhibitors from Boar Seminal Plasma and Boar Spermatozoa.- Proteinase Inhibitors in Human Cervical Mucus and Their in vitro Interactions with Human Acrosin.- Section III Pancreatic Proteinase Inhibitors.- Human Pancreatic Secretory Trypsin Inhibitor.- Isolation of Human Pancreatic Inhibitor and the Study of Its Interaction with Mammalian and Human Proteases.- Studies on Inhibition of the Two Human Trypsins.- Sequence of Cleavages in Temporary Inhibitors: Porcine Inhibitor Form II and Bovine Isoinhibitors A and C.- Temporary Inhibition — A Result of Consecutive Peptide Bond Hydrolysis.- Measurement of the Bovine Pancreatic Trypsin Inhibitors by Radioimmunoassay.- Properties and Inhibition Spectrum of a New Human Pancreatic Protease.- Section IV Characterization and Structure of New Inhibitors.- Amino Acid Sequence of Snail Inhibitor K and Correlation of Structure and Specificity.- Novel Proteinase Inhibitors in Snake Venoms: Distribution, Isolation, and Amino Acid Sequence.- Broad Specificity Inhibitors from Sea Anemones.- Discussion Remark: A Polyvalent Proteinase Inhibitor in the Earthworm (Lumbricus terrestris).- Discussion Remark: Proteinase Isoinhibitors from Cuttle Fish (Loligo vul-garis) H.Tschesche.- The Partial Linear Sequence of Garden Bean Inhibitor II and Location of the Protease Reactive Sites.- Elastase Inhibitors as Impurities in Commercial Preparations of Soybean Trypsin Inhibitor (Kunitz).- A New Alkaline Proteinase Inhibitor from Streptomyces albogriseolus.- Section V Structure-Function Relationships.- Replacements, Insertions, and Modifications of Amino Acid Residues in the Reactive Site of Soybean Trypsin Inhibitor (Kunitz).- Chemical Structure and Inhibitory Activities of Soybean Proteinase Inhibitors.- Structure-Function Relationships in Lima Bean Protease Inhibitor.- Structure-Activity Relationships of Several Trypsin and Chymotrypsin Inhibitors from Legume Seeds.- Reactive Site Cleavage, Thermodynamic Control Resynthesis, and Properties of Chemically Derivatized Trypsin-Kallikrein Inhibitors.- The Interaction of Catalytically Inactive Proteolytic Enzymes with Protein Inhibitors. Methyl-Chymotrypsin.- Discussion Remark: Inhibition of Anhydro-Trypsin and Anhydro- Chymotrypsin.- Section VI Physical-Chemical Studies of Enzyme-Inhibitor Interactions.- Kinetics and Thermodynamics of the Interaction of Proteinases with Protein Inhibitors.- Kinetic Studies of the Binding of Bovine Basic Pancreatic Trypsin Inhibitor to ?-Chymotrypsin.- The Mechanism of Association of Trypsin (or Chymotrypsin) with the Pancreatic Trypsin Inhibitors (Kunitz and Kazal), Kinetics and Thermodynamics of the Interaction.- Kinetic Studies on the Interactions between Native, Acetylated and Succinylated Trypsin and Natural Proteinase Inhibitors.- The Use of Fluorescence Techniques in the Study of the Interaction of the Basic Trypsin Inhibitor of Bovine Pancreas, Selectively Labelled at Lysine 15, with Chymotrypsin and Trypsin.- Raman Studies of Native and Partially (Cys 14-Cys 38) Reduced Basic Pancreatic Trypsin Inhibitor.- A Study on the Interaction of Trasylol with Trypsin and Kallikrein by Circular Dichroism.- Some Kinetic Consequences of the Tight Binding of Protein-Proteinase- Inhibitors to Proteolytic Enzymes and Their Application to the Determination of Dissociation Constants.- Section VII Molecular Aspects of Enzyme-Inhibitor Interactions.- Stereochemistry of Substrate Binding and Hydrolysis in the Trypsin Family of Enzymes.- The Model of the Basic Pancreatic Trypsin Inhibitor Refined at 1.5 Å Resolution.- Structure of the Complex Formed by Bovine Trypsin and Bovine Pancreatic Trypsin Inhibitor, Refinement of the Crystal Structure Analysis.- The Mode of Action of Soybean Trypsin Inhibitor as Revealed by Crystal Structure Analysis of the Complex with Porcine Trypsin.- Section VIII Organic Chemical Models and Low-Molecular-Weight Inhibitors.- Organic Chemical Models for Proteinase Inhibitors.- Progress in Designing Small Inhibitors Which Discriminate among Trypsin-Like Enzymes.- Homoarginine Oligomers as Trypsin and Chymotrypsin Substrates and Inhibitors.- Proteinase Inhibitors of Microbial Origin. A Review.- Discussion Remark: Low Molecular Weight Carboxydipeptidase Inhibitors from Bothropsjararaca Venom.- Section IX Inhibition of Non-Serine Proteases.- Carboxypeptidase Inhibitor from Potatoes. Properties and Amino Acid Sequence.- Inhibitors of Lysosomal Proteinases.- Inhibition of Insulin Specific Protease by Proteinase Inhibitors from the Snail (Helix pomatia).- An Insulin Degrading Proteinase from Human Erythrocytes and Its Inhibition by Proteinase Inhibitors.- Section X Evolution of Specificity of Proteinase Inhibitors.- Evolution of Specificity of Protein Proteinase Inhibitors.- Section XI Proteinases and Inhibitors in Biological Systems.- Proteases and Protease Inhibitors in Neoplasia.- Secretion of Enzymes by Neoplastic Cells and Macrophages.- Plasminogen Activators of Normal and Transformed Cells.- Proteinase Inhibitors from Ascaris lumbricoides: Properties and Their Physiological Role.- Wound-Induced Accumulation of Proteinase Inhibitor I in Tomato Leaves.- Section XII General Discussion Remark, Resumé, Concluding Remarks.- General Discussion Remark: Energetics of Inhibitor Binding.- Resumé.- Concluding Remarks.- Author Index.