Protein Nanotechnology: Protocols, Instrumentation, and Applications, Second Edition

ISBN-13: 9781627033534 / Angielski / Twarda / 2013 / 371 str.

Juliet A. Gerrard

Opis

Since the first edition of Protein Nanotechnology Protocols Instruments and Applications the intersection of protein science and nanotechnology has become an exciting frontier in interdisciplinary sciences. The second edition of Protein Nanotechnology Protocols Instruments and Applications expands upon the previous editions with current, detailed chapters that provide examples of proteins which are now being harnessed for a wide range of applications, some more developed than others. This book also delves into engineering proteins and an overview of the sorts of tools that are now readily available to manipulate the structure and function of proteins, both rationally and using methods inspired by evolution. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Protein Nanotechnology Protocols Instruments and Applications, Second Edition seeks to provide an overview of this multi-faceted field and a useful guide to those who wish to contribute to it.

Spis treści

1. Protein Nanotechnology – What is it?

Juliet A. Gerrard

 

Part I: Old Proteins, New Tricks

 2. Bioengineered Silk Proteins to Control Cell and Tissue Functions

Rucsanda C. Preda, Gary Leisk, Fiorenzo Omenetto, David L. Kaplan

 

3. Aqueous-based Spinning of Fibers from Self-Assembling Structural Proteins

Steven Arcidiacono, Elizabeth A. Welsh, Jason W. Soares

 

4. Fibrous Protein Nanofibres

Jeffrey E. Plowman, Santanu Deb-Choudhury, and Jolon M. Dyer

 

5. Self-assembling Nanomaterials: Monitoring the Formation of Amyloid Fibrils, with a Focus on Small Angle X-ray Scattering

Elizabeth B. Sawyer and Sally L. Gras

 

6. Amyloid Fibrils from Readily Available Sources: Milk Casein and Lens Crystallin Proteins

Heath Ecroyd, Megan Garvey, David C. Thorn, Juliet A. Gerrard, and John A. Carver

 

7. Formation of Amphipathic Amyloid Monolayers from Fungal Hydrophobin Proteins

Vanessa K. Morris and Margaret Sunde

 

8. Proteins and Peptides as Biological Nanowires: Towards Biosensing Devices

Laura J. Domigan

 

9. Nanotechnology with S-Layer Proteins

Bernhard Schuster and Uwe B. Sleytr

 

Part II: New Proteins

10. Stimuli-responsive Peptide Nanostructures at the Fluid-fluid Interface

Chun-Xia Zhao, Anton P.J. Middelberg

 

11. Designed Self-assembling Peptides as Templates for the Synthesis of Metal Nanoparticles

Emmanouil Kasotakis and Anna Mitraki

 12. Purification of Molecular Machines and Nanomotors using Phage-derived Monoclonal Antibody Fragments

Olga Esteban, Daniel Christ, and Daniela Stock

 

13. Determination of Enzyme Thermal Parameters for Rational Enzyme Engineering and Environmental/Evolutionary Studies

Charles K. Lee, Colin R. Monk, and Roy M. Daniel

Part III: Tools of the Trade14. Rational-Based Protein Engineering: Tips and ToolsMeghna Sobti and Bridget C. Mabbutt

 

15. Construction and Analysis of Randomized Protein-encoding Libraries using Error-prone PCR

Paulina Hanson-Manful and Wayne M. Patrick

 

16. Droplets as Reaction Compartments for Protein Nanotechnology

Sean R A Devenish, Miriam Kaltenbach, Martin Fischlechner, Florian Hollfelder

 

17. Label-Free, Real-Time Interaction and Adsorption Analysis 1: Surface Plasmon Resonance

Conan J. Fee

 

18. Label-Free, Real-Time Interaction and Adsorption Analysis 2: Quartz Crystal Microbalance

Conan J. Fee

 

19. Atomic Force Microscopy for Protein Nanotechnology

Dmitry V. Sokolov