Protein NMR Techniques

ISBN-13: 9781493961955 / Angielski / Miękka / 2016 / 518 str.

Alexander Shekhtman; David S. Burz

Opis

In its expanded third edition, this Methods in Molecular Biology (TM) volume offers techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing, materials lists, step-by-step protocols, troubleshooting tips and more.

Spis treści

Table of Contents

1. A Novel Bacterial Expression Method with Optimized Parameters for Very High Yield Production of Triple-labeled Proteins

2 Isotopic Labeling of Heterologous Proteins in the Yeast Pichia pastoris and Kluyveromyces lactis

3. Isotope Labeling in Insect Cells

4. Isotope Labeling in Mammalian Cells

5. Cell-free Protein Production for NMR Studies

6. Cell-free Membrane Protein Expression for Solid-state NMR

7. Expression and Purification of Src-family Kinases for Solution NMR Studies

8. NMR Studies of Large Protein Systems

9. Protein Dynamics by ¹⁵N Nuclear Magnetic Relaxation

10. Bacterial Production and Solution NMR Studies of a Viral Membrane Ion Channel

11. Preparation of the Modular Multi-Domain Protein RPA for Study by NMR Spectroscopy

12. NMR Studies of Protein-RNA Interactions

13. Preparation and Optimization of Protein-DNA Complexes Suitable for Detailed NMR Studies

14. NMR Studies of Protein-ligand Interactions

15. In-cell NMR Spectroscopy in Escherichia coli

16. Deuterated Peptides and Proteins: Structure and Dynamics Studies by MAS solid-state NMR

17. Solid-State NMR Spectroscopy of Protein Complexes

18. Synthesis, Purification and Characterization of Single Helix Membrane Peptides and Proteins for NMR Spectroscopy

19. Assignment of Backbone Resonances in a Eukaryotic Protein Kinase – ERK2

20. Electrostatics of Hydrogen Exchange for Analyzing Protein Flexibility

21. Fast Protein Backbone NMR Resonance Assignment using the BATCH Strategy

22. Comprehensive Automation for NMR Structure Determination of Proteins

 

23. ARIA for Solution and Solid-state NMR Protein NMR Techniques

24. Determining Protein Dynamics from ¹⁵N Relaxation Data by using DYNAMICS

 

Blurb

The field of protein NMR spectroscopy has rapidly expanded into new areas of biochemistry, molecular biology and cell biology research that were impossible to study as recently as ten years ago. This third edition of Protein NMR Techniques, expands upon the previous editions with current, detailed authoritative but down-to-earth descriptions of new methodologies. These include techniques for NMR sample preparation, solution and solid state NMR methodologies and data processing. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and practical, Protein NMR Techniques, Third Edition, seeks to aid scientists in understanding the latest innovations in the field of protein NMR.