Protein Microarrays: Methods and Protocols

ISBN-13: 9781617792854 / Angielski / Twarda / 2011 / 398 str.

Ulrike Korf

Opis

Progress in functional proteomics has been limited for a long time, partially caused by limitations in assay sensitivity and sample capacity; however, protein microarrays have the ability to overcome these limitations so that a highly parallel analysis of hundreds of proteins in thousands of samples is attainable. In Protein Microarrays: Methods and Protocols, expert researchers in the field present an up-to-date collection of robust strategies in the field of protein microarrays and summarize recent advantages in the field of printing technologies, the development of suitable surface materials, as well as detection and quantification technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by- step, readily reproducible laboratory protocols, and key notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Protein Microarrays: Methods and Protocols aims to stimulate the application and further advancement of this powerful technology in labs worldwide.

Spis treści

Part I: Reverse Phase Protein Arrays

 

1. Reverse Phase Protein Microarrays for Clinical Applications

            Mariaelena Pierobon, Claudio Belluco, and Lance A. Liotta, and Emanuel F. Petricoin III

 

2. Impact of Blocking and Detection Chemistries on Antibody Performance for Reverse Phase Protein Arrays

            Kristi Ambroz

 

3. Phosphoprotein Stability in Clinical Tissue and Its Relevance for Reverse Phase Protein Microarray Technology

            Virginia Espina, Claudius Mueller, and Lance A. Liotta

 

4. Utilization of RNAi to Validate Antibodies for Reverse Phase Protein Arrays

            Heiko Mannsperger, Stefan Uhlmann, Ulrike Korf, and Özgür Sahin

 

5. Antibody-Mediated Signal Amplification for Reverse Phase Protein Array-Based Protein Quantification

            Jan C. Brase, Heiko Mannsperger, Holger Sültmann, and Ulrike Korf

 

6. Reverse-Phase Protein Lysate Microarray (RPA) for the Experimental Validation of Quantitative Protein Network Models

            Satoshi Nishizuka

 

7. Characterization of Kinase Inhibitors Using Reverse Phase Protein Arrays

            Georg Martiny-Baron, Dorothea Haasen, Daniel D’Dorazio, Johannes Voshol, and Doriano Fabbro

 

8. Use of Formalin-Fixed and Paraffin-Embedded (FFPE) Tissues  for Diagnosis and Therapy in Routine Clinical Settings

            Daniela Berg, Katharina Malinowsky, Bilge Ergin, Claudia Wolff, and Karl-Friedrich Becker

 

9. Producing Reverse Phase Protein Microarrays from Formalin Fixed Tissues

            Claudia Wolff, Claudia Schott, Katharina Malinowsky, Daniela Berg, and Karl-Friedrich Becker

 

10. Use of Reverse Phase Protein Microarrays to Study Protein Expression in Leukemia: Technical and Methodological Lessons Learned

            Steven M. Kornblau and Kevin C. Coombes

 

Part II: Antibody Microarrays

 

11. Antibody Microarrays as Tools for Biomarker Discovery

            Marta Sanchez-Carbayo

 

12. Assessment of Antibody Specificity Using Suspension Bead Arrays

            Jochen M. Schwenk and Peter Nilsson

 

13. Quantitative Analysis of Phosphoproteins Using Microspot Immunoassays

            Frauke Henjes, Frank Götschel, Anika Jöcker, and Ulrike Korf

 

14. Robust Protein Profiling with Complex Antibody Microarrays in a Dual-Color Mode

            Christoph Schröder, Mohamed S.S. Alhamdani, Kurt Fellenberg, Andrea Bauer, Anette Jacob, and Jörg D. Hoheisel

 

15. High-Throughput Studies of Protein Glycoforms Using Antibody-Lectin Sandwich Arrays

            Brian B. Haab and Tingting Yue

 

16. Microspot Immunoassay-Based Analysis of Plasma Protein Profiles for Biomarker Discovery Strategies

            Johanna Sonntag, Heiko Mannsperger, Anika Joecker, and Ulrike Korf

 

17. Recombinant Antibodies for the Generation of Antibody Arrays

            Carl A.K. Borrebaeck and Christer Wingren

 

Part III: Protein Microarrays

 

18. Producing Protein Microarrays from DNA Microarrays

            Oda Stoevesandt, Michael J. Taussig, and Mingyue He

 

19. Cell Arrays and High-Content Screening

            Holger Erfle, Anastasia Eskova, Jürgen Reymann, and Vytaute Starkuviene

 

20. Probing Calmodulin Protein-Protein Interactions Using High Content Protein Arrays

            David J. O’Connell, Mikael Bauer, Sara Linse, and Dolores J. Cahill

 

21. Protein Function Microarrays for Customized Systems-Oriented Proteome Analysis

            Jonathan M. Blackburn and Aubrey Shoko

 

22. Optimized Autoantibody Profiling on Protein Arrays

            Sara L. O’Kane, John K. O’Brien, and Dolores J. Cahill

 

Part IV: Sample Immobilization Strategies

 

23. Inkjet Printing for the Production of Protein Microarrays

            Iain McWilliam, Marisa Chong Kwan, and Duncan Hall

 

24. Impact of Substrates for Probe Immobilization

            Ursula Sauer

 

25. Contact Printing of Protein Microarrays

            John Austin