Protein Engineering for Therapeutics, Part a: Volume 502

ISBN-13: 9780124160392 / Angielski / Twarda / 2012 / 392 str.

K. Dane Wittrup

Opis

This volume of Methods in Enzymology looks at Protein Engineering for Therapeutics. The chapters provide an invaluable resource for academics, researchers and students alike. With an international board of authors, this volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

• Chapters provide an invaluable resource for academics, researchers and students alike
• Iinternational board of authors
• This volume is split into sections that cover subjects such as Antibodies, Protein conjugates, Peptides, Enzymes and Scaffolds

Spis treści

1. Simplified Synthetic Antibody Libraries
2. Generation of Dual Variable Domain Immunoglobulin Molecules for Dual-specific Targeting
3. Discovery of internalizing antibodies to tumor antigens from phage libraries
4. Optimizing properties of anti-receptor antibodies using kinetic computational models and experiments
5. Cell-specific siRNA delivery by peptides and antibodies
6. Conjugation of Anticancer Drugs Through Endogenous Monoclonal Antibody Cysteine Residues
7. Building and characterizing antibody-targeted lipidic nanotherapeutics
8. Cell-Targeting Fusion Constructs Containing Recombinant Gelonin
9. Stapled Peptides for Intracellular Drug Targets
10. Mapping of vascular zip codes by phage display
11. Engineering cyclic peptide toxins
12. Peptide Discovery using Bacterial Display and Flow Cytometry
13. Oral enzyme therapy for celiac sprue
14. Rational Design and Evaluation of Mammalian Ribonuclease Cytotoxins
15. Engineering Reduced-Immunogenicity Enzymes for Amino Acid Depletion Therapy in Cancer
16. Designed Ankyrin Repeat Proteins (DARPins): From research to therapy
17. Target-binding proteins based on the tenth human fibronectin type III domain (¹⁰Fn3)
18. Anticalins: small engineered binding proteins based on the lipocalin scaffold
19. T Cell Receptor Engineering
20. Engineering Knottins as Novel Binding Agents
21. Re-Engineering Biopharmaceuticals for Targeted Delivery Across the Blood-Brain Barrier
22. Practical Theoretic Guidance for the Design of Tumor Targeting Agents
23. Fusions of elastin-like polypeptides to pharmaceutical proteins
24. Engineering and Identifying Supercharged Proteins for Macromolecule Delivery into Mammalian Cells