Protein Electrophoresis: Methods and Protocols

ISBN-13: 9781493962419 / Angielski / Miękka / 2016 / 648 str.

Biji T. Kurien; R. Hal Scofield

Opis

Each chapter in this Methods in Molecular Biology (TM) book details a specific electrophoretic variant, so that scientists can perform new techniques without difficulty. Includes step-by-step protocols, troubleshooting advice and tips on avoiding pitfalls.

Spis treści

1. How It All Began -- A Personal History of Gel-Electrophoresis

                 Oliver Smithies

 

2. Introduction to Protein Electrophoresis

                Pothur R. Srinivas

 

3. Protein Quantification Methods to Determine Protein Concentration Prior to Electrophoresis

                J.P. Dean Goldring

 

4. Protein Solubilization: Attend to the Choice of Lysis Buffer

                Mandy Peach, Noelle Marsh, and Daniel J. MacPhee

 

5. Native Polyacrylamide Gels

                Claudia Arndt, Stefanie Koristka, Holger Bartsch, and Michael Bachmann

 

6. Cationic Electrophoresis

                 Engelbert Buxbaum

 

7. SARCOSYL-PAGE: A New Electrophoretic Method for the Separation and Immunological Detection of PEGylated Proteins

                Christian Reichel

 

8. Tricine-SDS-PAGE

                Syed R. Haider, Helen J. Reid, and Barry L. Sharp

 

9. Analysis of Protein Glycation using Phenylboronate Acrylamide Gel Electrophoresis

                Marta P. Pereira Morais, John S. Fossey, Tony D. James, Jean M. H. van den Elsen

 

10. Protein Electrophoresis in Agarose Gels for Separating High Molecular Weight Proteins

                Marion L. Greaser and Chad M. Warren

 

11. Increase in Local Protein Concentration by Field-Inversion Gel Electrophoresis

                Henghang Tsai and Hon-chiu Eastwood Leung

 

12. A Combined Free Flow Electrophoresis and DIGE Approach to Compare Proteins in Complex Biological Samples

                Kim Y.C. Fung, Chris Cursaro, Tanya Lewanowitsch, Leah Cosgrove, and Peter Hoffmann

 

13. Immunofixation Electrophoresis for Identification of Proteins and Specific Antibodies

                Gyorgy Csako

 

14. Microchip Electrophoresis, with respect to “Profiling of Aβ Peptides in the Cerebrospinal Fluid of Patients with Alzheimer’s Disease”

                Mohamad Reza Mohamadi, Romain Verpillot, Myriam Taverna, Markus Otto, and Jean-Louis Viovy

 

15. A Foodomics Approach: CE-MS for Comparative Metabolomics of Colon Cancer Cells Treated with Dietary Polyphenols

                Mustafa Çelebier, Clara Ibáñez, Carolina Simó, and Alejandro Cifuentes

 

16. Peptide Fractionation by SDS-Free Polyacrylamide Gel Electrophoresis for Proteomic Analysis via DF-PAGE

                Yassel Ramos, Vladimir Besada, and Lila Castellanos-Serra

 

17. Tris-Acetate Polyacrylamide Gradient Gels for the Simultaneous Electrophoretic Analysis of Proteins of Very High and Low Molecular Mass

                Monica Cubillos-Rojas, Fabiola Amair-Pinedo, Irantzu Tato, Ramon Bartrons, Francesc Ventura, and Jose Luis Rosa

 

18. SDS PAGE for 35S Immunoprecipitation and Immunoprecipitation Western Blotting

                Edward P. Trieu and Ira N. Targoff

19. Two Dimensional Gel Electrophoresis: Vertical Isoelectric Focusing

                Yaser Dorri

 

20. Isoelectric Focusing in Agarose Gel for Detection of Oligoclonal Bands in Cerebrospinal and Other Biological Fluids

                Gyorgy Csako

 

21. Native Flat-Bed Isoelectric Focusing for Determining Antibody Clonotype Distribution

                Biji T. Kurien and R. Hal Scofield

 

22. Two-Dimensional Gel Electrophoresis: Glass Tube-Based IEF Followed by SDS-PAGE

                Hiroyuki Matsumoto, Hisao Hanju, Biji T. Kurien, and Naoka Komori

 

23. One-Dimensional and Two-Dimensional Immobilized Metal Affinity Electrophoresis

                Bao-Shiang Lee, G.D. Lasanthi, P. Jayathilaka, Jin-Sheng Huang, and Shalini Gupta 

 

24. Two-Dimensional Difference Gel Electrophoresis      

                Jonathan S. Minden

 

25. Protein Identification on Archived 2-D Gels

                Hiroyuki Matsumoto, Nobuaki Takemori, and Naoka Komori

 

26. Diagonal Electrophoresis for the Detection of Protein Disulfides

                Brian McDonagh

 

27. Two-Dimensional Blue Native/SDS Gel Electrophoresis of Multiprotein Complexes

                Jean Paul Lasserre and Armelle Ménard

 

28. Immunoelectrophoresis: A Method with Many Faces

                Gyorgy Csako

 

29. Cell Surface Protein Biotinylation for SDS PAGE Analysis

                Giuliano Elia

 

30. A Multichannel Gel Electrophoresis and Continuous Fraction Collection Apparatus for High-Throughput Protein Separation and Characterization

                Ming Dong, Megan Choi, Mark D. Biggin, and Jian Jin

 

31. Gel Absorption-Based Sample Preparation Method for Shotgun Analysis of Membrane Proteome

                Xianchun Wang and Songping Liang

 

32. Interactions of Hemoglobin in Live Red Blood Cells Measured by the Electrophoresis Release Test

                 Yan Su, Lijun Gao, Wenbin Qin

 

33. Extraction of Proteins from Gels - A Brief Review

                Biji T. Kurien and R. Hal Scofield

 

34. A New Algorithm for the Simulation of SDS 2D-PAGE Datasets

                Emilio Marengo and Elisa Robotti

 

35. Isolation of  Proteins from Polyacrylamide Gels

                Irene Michalk, Stefanie Koristka, Claudia Arndt, and Michael Bachmann

 

36. Gel Drying Methods

                Slava Stamova, Irene Michalk, Holger Bartsch, and Michael Bachmann

 

37. Long-Term, Buffer-Less Wet Gel Storage in Non-Sealed Polyethylene Bags

                Biji T. Kurien and R. Hal Scofield

 

38. A Brief Review of Other Notable Electrophoretic Methods

                James D. Fesmire

 

39. Protein Stains and Applications

                Ranjini K. Sundaram, Natarajan Balasubramaniyan, and Pazhani Sundaram

 

40. Coomassie-Brilliant Blue Staining of Polyacrylamide Gels

                Claudia Arndt, Stefanie Koristka, Anja Feldmann, Holger Bartsch, and Michael Bachmann

 

41. Accelerated Coomassie Blue Protein Staining and Destaining with Application of Heat

                Biji T. Kurien and R. Hal Scofield

 

42. Silver Staining Techniques of Polyacrylamide Gels

                Holger Bartsch, Claudia Arndt, Stefanie Koristka, Marc Cartellieri, and Michael Bachmann

 

43. Revisit of Imidazole-Zinc Reverse Stain for Protein Polyacrylamide Gel Electrophoresis

                Han-Min Chen

 

44. Counterion Dye Staining of Proteins in One- and Two-Dimensional Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis and Tryptic Gel Digestion of Stained Protein for Mass Spectrometry

                Jung-Kap Choi

 

45. TEMED Enhanced Photoluminescent Imaging of Human Serum Proteins by Quantum Dots after PAGE

                Na Na and Jin Ouyang

 

46. Microwave Assisted Protein Staining, De-Staining and In-Gel / In-Solution Digestion of Proteins

                Jennie R. Lill and Victor J. Nesatyy

 

47. Electrophoretic Separation and Detection of Metalloproteins by X-ray Fluorescence Mapping

                Tripti Khare, Yasmin Chishti, and Lydia A. Finney

 

48. Fluorescent Staining of Gels

                Engelbert Buxbaum

 

49. A Single-Step Simultaneous Protein Staining Procedure for Polyacrylamide Gels and Nitrocellulose Membranes by Alta During Western Blot Analysis

                Jayanta K. Pal, Sunil K. Berwal, and Rupali N. Soni

 

50. Detection of Glycoproteins in Polyacrylamide Gels Using Pro-Q Emerald 300 Dye, a Fluorescent Periodate Schiff-Base Stain

                Padmaja Mehta-D’souza

 

51. Spicy SDS-PAGE Gels: Curcumin/Turmeric as an Environment-Friendly Protein Stain                 

                Biji T. Kurien, Yaser Dorri, and R. Hal Scofield

 

52. In-Gel Staining of Proteins in Native Poly Acryl Amide Gel  Electrophoresis Using meso-Tetrakis(4-sulfonato phenyl)porphyrin

                K. Divakar, G. Nandhini Devi, and Pennathur Gautam

 

53. Rapid Detection of Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black

                J.P. Dean Goldring, Robert G.E. Krause, and David Choveaux

 

54. Zymographic Assay of Oxidases Using Peroxidase or Hemin Entrapped in Polyacrylamide Gels

                Mircea Alexandru Mateescu, Carmen Calinescu, Tien Canh Le, Rodolfo Federico, and Bruno Mondovi

 

55. Coomassie Brilliant Blue Removal/Disposal from Gel Destain and Used Gel Stain in an Environment-Friendly Manner

                Biji  T. Kurien and Yaser Dorri

 

56. A Brief Review of Other Notable Protein Detection Methods on Acrylamide Gels

                Biji  T. Kurien and R. Hal Scofield

 

57. Ultra-Sensitive Protein Detection and Imaging: Comparison of Lumitein™, ProteoSilver™, SYPRO® Ruby, and Coomassie® Brilliant Blue Gel Stains

                Guang Ren, Carl K. Okerberg, and Suresh T. Mathews

 

58. Common Artifacts and Mistakes Made in Electrophoresis

                Biji  T. Kurien and R. Hal Scofield

Blurb

Proteins are the functional units of the cellular machinery and they provide significant information regarding the molecular basis of health and disease. Therefore, techniques to separate and isolate the various proteins are critical to studying and understanding their functional characteristics. One of the widely used techniques for this purpose is electrophoresis. In Protein Electrophoresis: Methods and Protocols, contributions from experts in the field have been collected in order to provide practical guidelines to this complex study. Each chapter outlines a specific electrophoretic variant in detail so that laboratory scientists may perform a technique new to their lab without difficulty. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Protein Electrophoresis: Methods and Protocols seeks to serve laboratory scientists with well-honed, detailed methodologies in an effort to further our knowledge of this essential field.