Protein Discovery Technologies

ISBN-13: 9780367385385 / Angielski / Miękka / 2019 / 272 str.

Renata Pasqualini; Wadih Arap

Opis

Featuring contributions from leading experts, Protein Discovery Technologies provides comprehensive coverage of the principles, techniques, strategies, and broad range of applications of protein discovery. This volume presents in-depth discussions of various methods for protein discovery, including bioinformatics, interaction cloning, protein purification, phage display, non-primate models, and chemical targeting. It also explores biological themes through the examination of cell death, angiogenesis, hemostasis, development, signal transduction, transcriptional control, cell cycle control, neurobiology, and quality control.

Spis treści

Discovery of Vascular Permeability Factor (VPF, VEGF, VPF/VEGF, VEGF-A165). Tumor Necrosis Factor and Its Family Members. Blood–Brain Barrier Models for Investigating CNS Pathologies. Identification of Activation of Latent TGFß as Principal In Vivo Function of Integrin avß6. Purification of Basic Fibroblast Growth Factor by Heparin Affinity. How It Came to Pass that Interferon Was Used as an Angiogenesis Inhibitor. Use of Phage Display to Discover Inhibitors of Cell Migration. Combinatorial Mapping of Vascular Zip Codes by In Vivo Phage Display. Chasing Elusive Cellular Prion Protein Receptor. Discovery of Molecular Chaperone GRP78 and GRP94 Inducible by Endoplasmic Reticulum Stress. Origin of Sphingomyelin Pathway. Endotoxin-Induced Myocarditis: A New Pathophysiological Entity in Systemic Inflammatory Response Syndrome. Discovery of DOCK180 Superfamily of Exchange Factors. Discovery of Extracellular Matrix Degradome as Novel Endogenous Regulators of Angiogenesis and Tumor Growth. RING Finger Proteins as E3 Ubiquitin Ligases. Discovery of Chemical Nature of Cross-Links in Collagen: A Personal Retrospective. Discovery of SPARC as Prototype for Matricellular Proteins.

O autorze

Renata Pasqualini, Ph.D., is the Buchanan & Seeger Professor of Medicine and Cancer Biology at The University of Texas M. D. Anderson Cancer Center. She is an internationally recognized expert in vascular biology, metastasis, and angiogenesis. Dr. Pasqualini originally co-developed in vivo phage display, which enables combinatorial mapping of tissue and disease-specific molecular addresses in vivo, allowing for the development of ligand-directed targeted delivery of therapeutic and imaging agents.

Wadih Arap, M.D., Ph.D., is the Stringer Professor of Medicine and Cancer Biology and serves as an Attending Physician and Deputy Chairman of the Department of Genitourinary Medical Oncology at The University of Texas M. D. Anderson Cancer Center. He is also a member of the Board of Scientific Counselors of the National Cancer Institute.