Protein Design: Methods and Applications

ISBN-13: 9781493944088 / Angielski / Miękka / 2016 / 279 str.

Valentin Kohler

Opis

Protein Design: Method and Applications, Second Edition expands upon the previous edition with current, detailed ideas on how to approach a potential protein design project. With new chapters on metals as structure-forming elements and functional sites, the design and characterization of fluorinated proteins, top-down symmetric deconstruction and the design of protein libraries and novel or repurposed enzymes. Written in the highly successful Methods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and intuitive, Protein Design: Method and Applications, Second Edition provides a number of practical protocols and instructive reviews to aid in the creation of new experiments.

Spis treści

1. De novo Design of Stable α-helices

Alexander Yakimov, Georgy Rychkov, and Michael Petukhov

 

2. Design of Monomeric Water-soluble b-hairpin and β-sheet Peptides

M. Angeles Jiménez

 

3. Combination of Theoretical and Experimental Approaches for the Design and Study of Fibril-forming Peptides

Phanourios Tamamis, Emmanouil Kasotakis, Georgios Archontis, and Anna Mitraki

 

4. Posttranslational Incorporation of Non-canonical Amino Acids in the RNase S System by Semisynthetic Protein Assembly

Maika Genz and Norbert Sträter

 

5. Design, Synthesis, and Study of Fluorinated Proteins

Benjamin C. Buer and E. Neil G. Marsh

 

6. High-Quality Combinatorial Protein Libraries Using the Binary Patterning Approach

Luke H. Bradley

 

7. Methods for Library-Scale Computational Protein Design

Lucas B. Johnson, Thaddaus R. Huber, and Christopher D. Snow

 

8. Symmetric Protein Architecture in Protein Design: Top-Down Symmetric Deconstruction

Liam M. Longo and Michael Blaber

 

9. Identification of Protein Scaffolds for Enzyme Design using Scaffold Selection

André C. Stiel, Kaspar Feldmeier, and  Birte Höcker

 

10. Computational Design of Novel Enzymes without Cofactors

Matthew D. Smith, Alexandre Zanghellini, and Daniela Grabs

 

11. De novo Design of Peptide Scaffolds as Novel Pre-organised Ligands for Metal-ion Coordination

Aimee J. Gamble and Anna F. A. Peacock

 

12. Computational Design of Metalloproteins

Avanish S. Parmar, Douglas Pike, and Vikas Nanda

 

13. Incorporation of Modified and Artificial Cofactors into Naturally Occurring Protein Scaffolds

Koji Oohor and Takashi Hayashi

 

14. Computational Redesign of Metalloenzymes for Catalyzing New Reactions

Per Jr. Greisen and  Sagar D. Khare