Protein Chromatography: Methods and Protocols

ISBN-13: 9781493964109 / Angielski / Twarda / 2016 / 426 str.

Dermot Walls; Sinead T. Loughran

Opis

This second edition expands on the previous edition with new chapters that are suitable for newcomers, as well as more detailed chapters that cover protein stability and storage, avoiding proteolysis during chromatography, protein quantitation methods including immuno-qPCR, and the challenges that scale-up of production poses to the investigator. Many of the chapters also discuss generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins as a means to enhance their solubility and simplify their purification on an individual scale or in high-throughput systems. This book also provides readers with chapters that describe not just the more commonly used methods, but also recently developed approaches such as proteomic/mass spectrometric techniques and Lectin-based affinity chromatography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and thorough, Protein Chromatography: Methods and Protocols, Second Edition is a valuable resource for anyone who is interested in the field of protein chromatography.

Spis treści

Part I Overview Chapters

1. A Synopsis of Protein and their Purification
Dermot Walls, Gary Cooney, and Sinéad T. Loughran

2. Gel-Filtration Chromatography

Ciarán Ó’Fágáin, Philip M. Cummins, and Brendan F. O’Connor

3. Immunoaffinity Chromatography: Concepts and Applications
Jenny Fitzgerald, Paul Leonard, Elaine Darcy, Shikha Sharma, and Richard O’Kennedy

4. Avoiding Proteolysis During Protein Purification

Barry J. Ryan and Gary T. Henehan

5. Scale-Up of Protein Purification: Downstream Processing Issues

John Joseph Milne

6. Phage Display: A Powerful Technology for the Generation of High Specificity Affinity Reagents from Alternative Immune Sources

William J.J. Finlay, Laird Bloom, Joanne Grant, Edward Franklin, Deirdre Ní Shúilleabháin, and Orla Cunningham

7. Protein Stability: Enhancement and Measurement

Ciarán Ó’Fágáin

8. Tagging Recombinant Proteins to Enhance Solubility and Aid Purification

Sinéad T. Loughran and Dermot Walls

Part II Protocols Chapters

9. Storage and Lyophilisation of Pure Proteins

Ciarán Ó’Fágáin and Keith Colliton

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10. Differential Precipitation and Solubilization of Proteins
Barry J. Ryan and Gemma K. Kinsella

11. Ion-Exchange Chromatography: Basic Principles and Application

Philip M. Cummins, Keith D. Rochfort, and Brendan F. O’Connor

12. Protein Quantitation and Analysis of Purity

Eva M. Campion, Sinéad T. Loughran, and Dermot Walls

13. Purification of Proteins Fused to Maltose-Binding Protein

Mario Lebendiker and Tsafi Danieli

14. Purification of Poly-Histidine-Tagged Proteins

Sinéad T. Loughran, Ronan T. Bree, and Dermot Walls

15. Purification of Antibodies Using Affinity Chromatography
Elaine Darcy, Paul Leonard, Jenny Fitzgerald, Martin Danaher, Hui Ma, and Richard O’ Kennedy

16. Optimized Generation of High-Affinity, High-Specificity Single-Chain Fv Antibodies from Multi-Antigen Immunized Chickens

William J.J. Finlay, Laird Bloom, Sreeja Varghese, Bénédicte Autin, and Orla Cunningham

17. Measuring Protein-Protein Interactions Using Biacore

Paul Leonard, Stephen Hearty, Hui Ma, and Richard O’Kennedy

18. Hydrophobic Interaction Chromatography

Brendan F. O’Connor and Philip M. Cummins

19. Fast Protein Liquid Chromatography

Ashkan Madadlou, Siobhan O’Sullivan, and David Sheehan

20. Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems

Michael Henry and Paula Meleady

21. Strategies for the Purification of Membrane Proteins

Sinéad M. Smith

22. Antimicrobial Peptide Production and Purification

Srinivas Suda, Des Field, and Niall Barron 

23. Lectin Affiity Chromatography (LAC)
< ` Brendan F. O’Connor, Donal Monaghan, and Jonathan Cawley