Protein Cages: Protein Cages: Design, Structure, and Applications

ISBN-13: 9781071632215 / Angielski

Takafumi Ueno; Sierin Lim; Kelin Xia

Opis

This volume provides the latest methods for synthesis, structural analysis, and elucidation of the mechanism. Chapters guide readers through methods on protein cages for nanotechnology, analyze designed protein cages, determine the structures, and even perform theoretical analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.  Authoritative and cutting-edge, Protein Cages: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

Spis treści

 

PART I:          Design and Construction of Artificial Cages

1 Coiled-coil Protein Origami: Design, Isolation and Characterization

Žiga Strmšek, Jaka Snoj, Tadej Satler, and Roman Jerala

 

2. Artificial Protein Cages Assembled Via Gold Coordination

Karolina Majsterkiewicz, Izabela Stupka, Kinga Borzęcka-Solarz, Artur Biela, Szymon Gaweł, Monika Pasternak, and Jonathan Heddle

 

3. Reassembly Design of Ferritin Cages

Yu Liu and Guanghua Zhao

 

4. Protein Cages and Nanostructures Constructed from Protein Nanobuilding Blocks

Naoya Kobayashi and Ryoichi Arai

 

5. Preparation of Cage-like Micellar Assemblies of Engineered Hemoproteins

Koji Oohora and Takashi Hayashi

 

PART II: Functionalization of Protein Cages

6. Fabrication of Protein Macromolecular Frameworks (PMFs) and Their Application in Catalytic Materials

Masaki Uchida, Ekaterina Selivanovitch, Kimberly McCoy, and Trevor Douglas

 

7. Iron Accumulation in Ferritin

Akankshika Parida and Rabindra K. Behera

 

8. A Generalized Method for Metal Fixation in Horse Spleen L-Ferritin Cage

Basudev Maity and Takafumi Ueno

9. Dual Modification of Artificial Protein Cage

Norifumi Kawakami, Erika Nasu, and Kenji Miyamoto

 

10.  Modification and Production of Encapsulin

Sandra Michel-Souzy and Jeroen J.L.M. Cornelissen

 

 

Part III: Characterization and Simulation of Protein Cage Systems

11. Cryo-electron Microscopy of Protein Cages

Raymond N. Burton-Smith and Kazuyoshi Murata

 

12. Time-resolved Small-angle X-Ray Scattering of Protein Cage Assembly

Daisuke Sato, Takaaki Hikima, and Masamichi Ikeguchi

 

13. Protein Cage-Stabilized Emulsions: Formulation and Characterization

Mridul Sarker, Samuel Watts, Stefan Salentinig, and Sierin Lim

 

14. A Method to Investigate the Mechanism of Charge Transport across Bio-Molecular Junctions with Ferritin Senthil Kumar Karuppannan and Christian A. Nijhuis

 

15. Potato Virus X Inactivation and Characterization

Yifeng Ma and Nicole F. Steinmetz

 

16. Molecular Dynamics Simulation of Protein Cages

Chenlin Lu, Xue Peng, and Diannan Lu

 

17. Coarse-grained Models for Vault Normal Model Analysis

D Vijay Anand, Ronald Koh Joon Wei, and Kelin Xia

 

Part IV: Translation of Protein Cages

18 . Evaluating Anti-Tumor Immune Responses of Protein Nanoparticle-Based Cancer Vaccines

Enya Li, Nina Butkovich, Jo A. Tucker, Edward L. Nelson, and Szu-Wen Wang

 

19. Construction Protocol of Drug-Protein Cage Complexes for Drug Delivery System

Yuichi Nakahara, Yuta Endo, and Ippei Inoue

 

20. Protein Cage Relaxivity Measurement for Magnetic Resonance Imaging Contrast Agents

Ambrish Kumar, Bhargy Sharma, and Sierin Lim

 

21. Crystalline Biohybrid Materials Based on Protein Cages

Hendrik Böhler, Michael Rütten, Laurin Lang, and Tobias Beck

 

22. Production and Purification of Virus-like Particles by Transient Expression in Plants

Lygie Esquirol, Donna McNeale, Micol Venturi, and Frank Sainsbury

 

23. Laboratory Scale Production of Complex Proteins Using Charge Complimentary Nanoenvironments

Girish Vallerinteavide Mavelli, Samira Sadeghi, and Chester Lee Drum

Blurb

This volume provides the latest methods for synthesis, structural analysis, and elucidation of the mechanism. Chapters guide readers through methods on protein cages for nanotechnology, analyze designed protein cages, determine the structures, and even perform theoretical analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 

Authoritative and cutting-edge, Protein Cages: Methods and Protocols aims to be a useful and practical guide. This volume provides the latest methods for synthesis, structural analysis, and elucidation of the mechanism. e to new researchers and experts looking to expand their knowledge.