Protein Cages: Methods and Protocols

ISBN-13: 9781493946709 / Angielski / Miękka / 2016 / 190 str.

Brendan P. Orner

Opis

This volume emphasizes new techniques to help understand protein cages and to apply them to a variety of technologies, highlighting the expertise of researchers based on three continents. Protein cages are currently inspiring diverse scientific disciplines and are therefore at the crossroads of extremely widely-scoped research, which is reflected in the detailed chapters of Protein Cages: Methods and Protocols. From nanomaterials studies and iron particles to computational strategies and Atomic Force Microscopy, the chapters herein collectively provide an introduction to the rich world of protein cage research and specific techniques to understand and exploit this fascinating class of proteins. Written in the highly successful Methods in Molecular Biology series format, chapters begin with an introduction to their respective topics, lists of the necessary materials, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and cutting-edge, Protein Cages: Methods and Protocols will help to inspire and further propel the current multi-disciplinary enthusiasm in studying and discovering new applications for protein cages.

Spis treści

1. Encapsulation of Nanoparticles in Virus Protein Shells

            Irina B. Tsvetkova and Bogdan G. Dragnea

 

2. Use of Protein Cages as a Template for Confined Synthesis of Inorganic and Organic Nanoparticles

            Masaki Uchida, Shefah Qazi, Ethan Edwards, and Trevor Douglas

 

3. Ferritin Encapsulation and Templated Synthesis of Inorganic Nanoparticles

            Katherine W. Pulsipher and Ivan J. Dmochowski

 

4. Determining the Relaxivity Values of Protein Cage-Templated Nanoparticles Using Magnetic Resonance Imaging

            Barindra Sana and Sierin Lim

 

5. Computationally Assisted Engineering of Protein Cages

            Maziar S. Ardejani and Brendan P. Orner

 

6. Recombinant Expression and Purification of “Virus-Like” Bacterial Encapsulin Protein Cages

            W. Frederik Rurup, Jeroen J.L.M. Cornelissen, and Melissa S.T. Koay

 

7. Production of Bacterial Microcompartments

            Jonathan K. Lassila

 

8. Detection of Protein Cage Assembly With Bis-Arsenic Fluorescent Probes

            Thomas A. Cornell and Brendan P. Orner

 

9. Determining the Role of Metal Binding in Protein Cage Assembly

            Anne Grove, Ambuj K. Kushwaha, and Khoa H. Nguyen

 

10. Differential Scanning Calorimetry to Quantify the Stability of Protein Cages

            Yu Zhang and Maziar S. Ardejani

 

11. Material Properties of Viral Nanocages Explored by Atomic Force Microscopy

            Mariska G.M. van Rosmalen, Wouter H. Roos, and Gijs J.L. Wuite

 

12. Computational Mechanics of Viral Capsids

            Melissa M. Gibbons, Luigi E. Perotti, and William S. Klug